| Autor: |
Filippova KO; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, Russia., Ermakov AM; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, Russia.; Moscow Region Pedagogical State University, Moscow 141014, Russia., Popov AL; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, Russia.; Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow 119991, Russia., Ermakova ON; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, Russia., Blagodatsky AS; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, Russia., Chukavin NN; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, Russia.; Moscow Region Pedagogical State University, Moscow 141014, Russia., Shcherbakov AB; Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 03680 Kyiv, Ukraine., Baranchikov AE; Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow 119991, Russia., Ivanov VK; Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow 119991, Russia. |
| Abstrakt: |
Novel radioprotectors are strongly demanded due to their numerous applications in radiobiology and biomedicine, e.g., for facilitating the remedy after cancer radiotherapy. Currently, cerium-containing nanomaterials are regarded as promising inorganic radioprotectors due to their unrivaled antioxidant activity based on their ability to mimic the action of natural redox enzymes like catalase and superoxide dismutase and to neutralize reactive oxygen species (ROS), which are by far the main damaging factors of ionizing radiation. The freshwater planarian flatworms are considered a promising system for testing new radioprotectors, due to the high regenerative potential of these species and an excessive amount of proliferating stem cells (neoblasts) in their bodies. Using planarian Schmidtea mediterranea , we tested CeO 2 nanoparticles, well known for their antioxidant activity, along with much less studied CeF 3 nanoparticles, for their radioprotective potential. In addition, both CeO 2 and CeF 3 nanoparticles improve planarian head blastema regeneration after ionizing irradiation by enhancing blastema growth, increasing the number of mitoses and neoblasts' survival, and modulating the expression of genes responsible for the proliferation and differentiation of neoblasts. The CeO 2 nanoparticles' action stems directly from their redox activity as ROS scavengers, while the CeF 3 nanoparticles' action is mediated by overexpression of "wound-induced genes" and neoblast- and stem cell-regulating genes. |
