| Autor: |
Peixoto RF; Immunology of Infectious Diseases Laboratory, Department of Cellular and Molecular Biology, Federal University of Paraiba, João Pessoa 58051-900, Brazil., Gois BM; Immunology of Infectious Diseases Laboratory, Department of Cellular and Molecular Biology, Federal University of Paraiba, João Pessoa 58051-900, Brazil., Martins M; Immunology of Infectious Diseases Laboratory, Department of Cellular and Molecular Biology, Federal University of Paraiba, João Pessoa 58051-900, Brazil., Palmeira PHS; Immunology of Infectious Diseases Laboratory, Department of Cellular and Molecular Biology, Federal University of Paraiba, João Pessoa 58051-900, Brazil., Rocha JC; Immunology of Infectious Diseases Laboratory, Department of Cellular and Molecular Biology, Federal University of Paraiba, João Pessoa 58051-900, Brazil., Gomes JAS; Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Pampulha, Belo Horizonte 31270-901, Brazil., Azevedo FLAA; Research Institute for Drugs and Medicines, Federal University of Paraíba, João Pessoa 58051-900, Brazil., Veras RC; Research Institute for Drugs and Medicines, Federal University of Paraíba, João Pessoa 58051-900, Brazil., de Medeiros IA; Research Institute for Drugs and Medicines, Federal University of Paraíba, João Pessoa 58051-900, Brazil., Grisi TCSL; Department of Biotechnology, Federal University of Paraiba, João Pessoa 58051-900, Brazil., de Araújo DAM; Department of Biotechnology, Federal University of Paraiba, João Pessoa 58051-900, Brazil., Amaral IPG; Immunology of Infectious Diseases Laboratory, Department of Cellular and Molecular Biology, Federal University of Paraiba, João Pessoa 58051-900, Brazil., Keesen TSL; Immunology of Infectious Diseases Laboratory, Department of Cellular and Molecular Biology, Federal University of Paraiba, João Pessoa 58051-900, Brazil. |
| Abstrakt: |
High IL-10 levels are pivotal to parasite survival in visceral leishmaniasis (VL). Antigenic stimuli induce IL-10 expression and release of adenosine by CD39/CD73. Due their intrinsic ability to express IL-10 and produce adenosine from extracellular ATP, we evaluated the IL-10, CD39, and CD73 expression by Regulatory T cells (Treg) correlated with VL pathology. Using flow cytometry, Treg cells was analyzed in peripheral blood samples from VL patients (in the presence and absence of Leishmania infantum soluble antigen (SLA)) and healthy individuals (negative endemic control-NEC group), without any treatment. Additionally, IL-10 levels in leukocytes culture supernatant were measured in all groups by ELISA assay. VL patients presented more Treg frequency than NEC group, independently of stimulation. ELISA results demonstrated that SLA induced higher IL-10 expression in the VL group. However, the NEC group had a higher Treg IL-10 + compared to the VL group without stimulation and SLA restored the IL-10 in Treg. Additionally, an increase in Treg CD73 + in the VL group independently of stimuli compared to that in the NEC group was observed. We suggest that Treg are not the main source of IL-10, while the CD73 pathway may be an attempt to modulate the exacerbation of immune response in VL disease. |
