Histone H2Bub dynamics in the 5' region of active genes are tightly linked to the UV-induced transcriptional response.

Autor:	Fanourgakis S; Institute for Fundamental Biomedical Research, BSRC 'Alexander Fleming', 16672 Vari, Attica, Greece., Synacheri AC; Institute for Fundamental Biomedical Research, BSRC 'Alexander Fleming', 16672 Vari, Attica, Greece., Lavigne MD; Institute for Fundamental Biomedical Research, BSRC 'Alexander Fleming', 16672 Vari, Attica, Greece., Konstantopoulos D; Institute for Fundamental Biomedical Research, BSRC 'Alexander Fleming', 16672 Vari, Attica, Greece., Fousteri M; Institute for Fundamental Biomedical Research, BSRC 'Alexander Fleming', 16672 Vari, Attica, Greece.
Jazyk:	angličtina
Zdroj:	Computational and structural biotechnology journal [Comput Struct Biotechnol J] 2022 Dec 13; Vol. 21, pp. 614-629. Date of Electronic Publication: 2022 Dec 13 (Print Publication: 2023).
DOI:	10.1016/j.csbj.2022.12.013
Abstrakt:	The timing and location of writing and erasing of histone modifications determine gene expression programs and are tightly controlled processes. One such modification is the monoubiquitination of histone H2B (H2Bub), whose precise level during transcription elongation is dynamically regulated by the synergistic action of RNF20/40 ubiquitin-ligase and the de-ubiquitinase (DUB) of the ATXN7L3-containing DUB modules. Here, we characterize the dynamics of H2Bub in transcription and explore its role in perspective with the recently updated model of UV damage-induced transcription reorganization. Employing integrative analysis of genome-wide high-throughput approaches, transcription inhibitors and ATXN7L3-DUB knockdown cells, we find that H2Bub levels and patterns depend on intron-exon architecture both in steady state and upon UV. Importantly, our analysis reveals a widespread redistribution of this histone mark, rather than a uniform loss as previously suggested, which closely mirrors the post-UV dynamics of elongating RNA Polymerase II (RNAPII) at transcribed loci. The observed effects are due to a direct inter-dependence on RNAPII local concentration and speed, and we show that deficient ATXN7L3-mediated DUB activity leads to increased elongation rates in both non-irradiated and irradiated conditions. Our data and the implementation of a high-resolution computational framework reveal that the H2Bub pattern follows that of RNAPII, both in the ATXNL3 knockdown and in response to UV guaranteeing faithful elongation speed, especially in the context of the transcription-driven DNA damage response. Competing Interests: The authors declare no conflict of interest. (© 2022 The Authors.)
Databáze:	MEDLINE
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