Advances in fluorescence microscopy for orthohantavirus research.

Autor:	Menke L; Nanoscale Infection Biology, Helmholtz Centre for Infection Research, Inhoffenstr. 7, Braunschweig 38124, Germany., Sperber HS; Institute for Translational HIV Research, University Hospital Essen, Virchowstr. 171, Essen 45147, Germany., Aji AK; Department of Physical Biochemistry, University of Potsdam, Institute of Biochemistry and Biology, Karl-Liebknecht-Str. 24-25, Potsdam 14476, Germany., Chiantia S; Department of Physical Biochemistry, University of Potsdam, Institute of Biochemistry and Biology, Karl-Liebknecht-Str. 24-25, Potsdam 14476, Germany., Schwarzer R; Institute for Translational HIV Research, University Hospital Essen, Virchowstr. 171, Essen 45147, Germany., Sieben C; Nanoscale Infection Biology, Helmholtz Centre for Infection Research, Inhoffenstr. 7, Braunschweig 38124, Germany.; Institute of Genetics, Technische Universität Braunschweig, Spielmannstr. 7, Braunschweig 38106, Germany.
Jazyk:	angličtina
Zdroj:	Microscopy (Oxford, England) [Microscopy (Oxf)] 2023 Jun 08; Vol. 72 (3), pp. 191-203.
DOI:	10.1093/jmicro/dfac075
Abstrakt:	Orthohantaviruses are important zoonotic pathogens responsible for a considerable disease burden globally. Partly due to our incomplete understanding of orthohantavirus replication, there is currently no effective antiviral treatment available. Recently, novel microscopy techniques and cutting-edge, automated image analysis algorithms have emerged, enabling to study cellular, subcellular and even molecular processes in unprecedented detail and depth. To date, fluorescence light microscopy allows us to visualize viral and cellular components and macromolecular complexes in live cells, which in turn enables the study of specific steps of the viral replication cycle such as particle entry or protein trafficking at high temporal and spatial resolution. In this review, we highlight how fluorescence microscopy has provided new insights and improved our understanding of orthohantavirus biology. We discuss technical challenges such as studying live infected cells, give alternatives with recombinant protein expression and highlight future opportunities, for example, the application of super-resolution microscopy techniques, which has shown great potential in studies of different cellular processes and viral pathogens. (© The Author(s) 2023. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu