Beneficial Bacteria and Plant Extracts Promote Honey Bee Health and Reduce Nosema ceranae Infection.

Autor: Garrido PM; Instituto de Investigaciones en Producción Sanidad y Ambiente (IIPROSAM), CONICET, UNMdP, Centro Asoc. Simple CIC PBA, Funes 3350, Mar del Plata, Buenos Aires, 7600, Argentina.; Centro de Investigación en Abejas Sociales (CIAS), FCEyN, UNMdP, Funes 3350, Mar del Plata, Buenos Aires, 7600, Argentina., Porrini MP; Instituto de Investigaciones en Producción Sanidad y Ambiente (IIPROSAM), CONICET, UNMdP, Centro Asoc. Simple CIC PBA, Funes 3350, Mar del Plata, Buenos Aires, 7600, Argentina.; Centro de Investigación en Abejas Sociales (CIAS), FCEyN, UNMdP, Funes 3350, Mar del Plata, Buenos Aires, 7600, Argentina., Alberoni D; Department of Agricultural and Food Sciences, University of Bologna, Viale Fanin 44, Bologna, 40127, Italy. daniele.alberoni@unibo.it., Baffoni L; Department of Agricultural and Food Sciences, University of Bologna, Viale Fanin 44, Bologna, 40127, Italy., Scott D; ADVANCE SCIENCE Ltd, Knocknacarra Rd, Galway, H91 XV84, Ireland., Mifsud D; Institute of Earth Systems, L-Università ta' Malta, University Ring Rd, Msida, MSD2080, Malta., Eguaras MJ; Instituto de Investigaciones en Producción Sanidad y Ambiente (IIPROSAM), CONICET, UNMdP, Centro Asoc. Simple CIC PBA, Funes 3350, Mar del Plata, Buenos Aires, 7600, Argentina.; Centro de Investigación en Abejas Sociales (CIAS), FCEyN, UNMdP, Funes 3350, Mar del Plata, Buenos Aires, 7600, Argentina., Di Gioia D; Department of Agricultural and Food Sciences, University of Bologna, Viale Fanin 44, Bologna, 40127, Italy.
Jazyk: angličtina
Zdroj: Probiotics and antimicrobial proteins [Probiotics Antimicrob Proteins] 2024 Feb; Vol. 16 (1), pp. 259-274. Date of Electronic Publication: 2023 Jan 13.
DOI: 10.1007/s12602-022-10025-7
Abstrakt: The research aims to give new insights on the effect of administering selected bacterial strains, isolated from honey bee gut, and/or a commercial plant extract blend (HiveAlive ® ) on Nosema ceranae. Analyses were first performed under laboratory conditions such as different infective doses of N. ceranae, the effect of single strains and their mixture and the influence of pollen administration. Daily survival and feed consumption rate were recorded and pathogen development was analysed using qPCR and microscope counts. Biomarkers of immunity and physiological status were also evaluated for the different treatments tested using one bacterial strain, a mixture of all the bacteria and/or a plant extract blend as treatments. The results showed an increase of abaecin transcript levels in the midgut of the honey bees treated with the bacterial mixture and an increased expression of the protein vitellogenin in the haemolymph of honey bees treated with two separate bacterial strains (Bifidobacterium coryneforme and Apilactobacillus kunkeei). A significant effectiveness in reducing N. ceranae was shown by the bacterial mixture and the plant extract blend regardless of the composition of the diet. This bioactivity was seasonally linked. Quantitative PCR and microscope counts showed the reduction of N. ceranae under different experimental conditions. The antiparasitic efficacy of the treatments at field conditions was studied using a semi-field approach which was adapted from research on insecticides for the first time, to analyse antiparasitic activity against N. ceranae. The approach proved to be reliable and effective in validating data obtained in the laboratory. Both the mixture of beneficial bacteria and its association with Hive Alive ® are effective in controlling the natural infection of N. ceranae in honey bee colonies.
(© 2023. The Author(s).)
Databáze: MEDLINE
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