| Autor: |
Thompson CD; Center for Immunology and Microbial Research, Department of Cell and Molecular Biology, The University of Mississippi Medical Center, Jackson, Mississippi, USA., Bradshaw JL; Center for Immunology and Microbial Research, Department of Cell and Molecular Biology, The University of Mississippi Medical Center, Jackson, Mississippi, USA., Miller WS; Mississippi INBRE Research Scholar Program, Mississippi College, Clinton, Mississippi, USA., Vidal AGJ; Center for Immunology and Microbial Research, Department of Cell and Molecular Biology, The University of Mississippi Medical Center, Jackson, Mississippi, USA., Vidal JE; Center for Immunology and Microbial Research, Department of Cell and Molecular Biology, The University of Mississippi Medical Center, Jackson, Mississippi, USA., Rosch JW; Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA., McDaniel LS; Center for Immunology and Microbial Research, Department of Cell and Molecular Biology, The University of Mississippi Medical Center, Jackson, Mississippi, USA., Keller LE; Center for Immunology and Microbial Research, Department of Cell and Molecular Biology, The University of Mississippi Medical Center, Jackson, Mississippi, USA. |
| Abstrakt: |
Streptococcus pneumoniae colonizes the human nasopharynx and causes several diseases. Pneumococcal vaccines target the polysaccharide capsule and prevent most serious disease, but there has been an increase in the prevalence of nonencapsulated S. pneumoniae (NESp). Previously, it was thought that a capsule was necessary to cause invasive disease. NESp strains expressing the oligopeptide transporters AliC and AliD have been isolated from patients with invasive disease. The AliC and AliD oligopeptide transporters regulate the expression of several genes, including choline binding protein AC (CbpAC) (a homolog of PspA), which aids in reducing C3b deposition. It is hypothesized that by altering CbpAC expression, AliC and AliD provide protection from classical complement-mediated clearance by reducing C-reactive protein (CRP) binding. Our study demonstrates that AliC and AliD regulate CbpAC expression in NESp and that AliD found in certain serotypes of encapsulated strains regulates PspA expression. C3b deposition was increased in the NESp Δ aliD and encapsulated mutants in comparison to the wild type. NESp strains expressing AliC and AliD have a significant decrease in C1q and CRP deposition in comparison to the Δ aliC Δ aliD mutant. The complement protein C1q is required for NESp clearance in a murine model and increases opsonophagocytosis. By regulating CbpAC expression, NESp inhibits CRP binding to the bacterial surface and blocks classical complement activation, leading to greater systemic survival and virulence. Due to the increase in the prevalence of NESp, it is important to gain a better understanding of NESp virulence mechanisms that aid in establishing disease and persistence within a host by avoiding clearance by the immune system. IMPORTANCE Streptococcus pneumoniae (pneumococcus) can cause a range of diseases. Although there is a robust pneumococcal vaccination program that reduces invasive pneumococcal disease by targeting various polysaccharide capsules, there has been an increase in the isolation of nonvaccine serotypes and nonencapsulated S. pneumoniae (NESp) strains. While most studies of pneumococcal pathogenesis have focused on encapsulated strains, there is little understanding of how NESp causes disease. NESp lacks a protective capsule but contains novel genes, such as aliC and aliD , which have been shown to regulate the expression of numerous genes and to be required for NESp virulence and immune evasion. Furthermore, NESp strains have high transformation efficiencies and harbor resistance to multiple drugs. This could be deleterious to current treatment strategies employed for pneumococcal disease as NESp can be a reservoir of drug resistance genes. Therefore, deciphering how NESp survives within a host and facilitates disease is a necessity that will allow the fabrication of improved, broad-spectrum treatments and preventatives against pneumococcal disease. Our study provides a better understanding of NESp virulence mechanisms during host-pathogen interactions through the examination of genes directly regulated by the NESp proteins AliC and AliD. |
