| Autor: |
Rogala P; Institute of Chemistry, Jan Kochanowski University of Kielce, 7 Uniwersytecka Str., 25-406 Kielce, Poland., Jabłońska-Wawrzycka A; Institute of Chemistry, Jan Kochanowski University of Kielce, 7 Uniwersytecka Str., 25-406 Kielce, Poland.; Faculty of Chemistry, Jagiellonian University, 2 Gronostajowa Str., 30-387 Kraków, Poland., Czerwonka G; Institute of Biology, Jan Kochanowski University of Kielce, 7 Uniwersytecka Str., 25-406 Kielce, Poland., Kazimierczuk K; Faculty of Chemistry, Gdańsk University of Technology, 11/12 G. Narutowicza Str., 80-233 Gdańsk, Poland., Gałczyńska K; Institute of Biology, Jan Kochanowski University of Kielce, 7 Uniwersytecka Str., 25-406 Kielce, Poland., Michałkiewicz S; Institute of Chemistry, Jan Kochanowski University of Kielce, 7 Uniwersytecka Str., 25-406 Kielce, Poland., Kalinowska-Tłuścik J; Faculty of Chemistry, Jagiellonian University, 2 Gronostajowa Str., 30-387 Kraków, Poland., Karpiel M; Faculty of Chemistry, Jagiellonian University, 2 Gronostajowa Str., 30-387 Kraków, Poland., Klika KD; Molecular Structure Analysis, NMR Spectroscopy Analysis Unit, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany. |
| Abstrakt: |
Half-sandwich Ru(II) complexes belong to group of biologically active metallo-compounds with promising antimicrobial and anticancer activity. Herein, we report the synthesis and characterization of arene ruthenium complexes containing benzimidazole moiety, namely, [(η 6 - p -cymene)RuCl(bimCOO)] ( 1 ) and [(η 6 - p -cymene)RuCl 2 (bim)] ( 2 ) (where bimCOO = benzimidazole-2-carboxylate and bim = 1- H -benzimidazole). The compounds were characterized by 1 H NMR, 13 C NMR, IR, UV-vis and CV. Molecular structures of the complexes were determined by SC-XRD analysis, and the results indicated the presence of a pseudo -tetrahedral (piano stool) geometry. Interactions in the crystals of the Ru complexes using the Hirshfeld surface analysis were also examined. In addition, the biological studies of the complexes, such as antimicrobial assays (against planktonic and adherent microbes), cytotoxicity and lipophilicity, were performed. Antibacterial activity of the complexes was evaluated against S. aureus , E. coli , P. aeruginosa PAO1 and LES B58. Cytotoxic activity was tested against primary human fibroblasts and adenocarcinoma human alveolar basal epithelial cells. Obtained biological results show that the ruthenium compounds have bacteriostatic activity toward Pseudomonas aeruginosa PAO1 strain and are not toxic to normal cells. A molecular docking study was applied as a predictive source of information about the plausibility of examined structures binding with HSA as a transporting system. |
