Rational design of magnetoliposomes for enhanced interaction with bacterial membrane models.

Autor: Soares FA; LAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade Do Porto, R. Jorge de Viterbo Ferreira 228, Porto, 4050-313, Portugal; ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade Do Porto, R. Jorge de Viterbo Ferreira 228, Porto, 4050-313, Portugal., Costa P; LAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade Do Porto, R. Jorge de Viterbo Ferreira 228, Porto, 4050-313, Portugal., Sousa CT; IFIMUP, Departamento de Física e Astronomia da Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre 687, Porto, 4169-007, Portugal., Horta M; LAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade Do Porto, R. Jorge de Viterbo Ferreira 228, Porto, 4050-313, Portugal., Pereira-Leite C; LAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade Do Porto, R. Jorge de Viterbo Ferreira 228, Porto, 4050-313, Portugal., Seabra CL; LAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade Do Porto, R. Jorge de Viterbo Ferreira 228, Porto, 4050-313, Portugal., Lima SAC; LAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade Do Porto, R. Jorge de Viterbo Ferreira 228, Porto, 4050-313, Portugal., Reis S; LAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade Do Porto, R. Jorge de Viterbo Ferreira 228, Porto, 4050-313, Portugal., Nunes C; LAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade Do Porto, R. Jorge de Viterbo Ferreira 228, Porto, 4050-313, Portugal; ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade Do Porto, R. Jorge de Viterbo Ferreira 228, Porto, 4050-313, Portugal. Electronic address: cdnunes@ff.up.pt.
Jazyk: angličtina
Zdroj: Biochimica et biophysica acta. Biomembranes [Biochim Biophys Acta Biomembr] 2023 Mar; Vol. 1865 (3), pp. 184115. Date of Electronic Publication: 2023 Jan 02.
DOI: 10.1016/j.bbamem.2022.184115
Abstrakt: There is a growing need for alternatives to target and treat bacterial infection. Thus, the present work aims to develop and optimize the production of PEGylated magnetoliposomes (MLPs@PEG), by encapsulating superparamagnetic iron oxide nanoparticles (SPIONs) within fusogenic liposomes. A Box-Behnken design was applied to modulate size distribution variables, using lipid concentration, SPIONs amount and ultrasonication time as independent variables. As a result of the optimization, it was possible to obtain MLPs@PEG with a mean size of 182 nm, with polydispersity index (PDI) of 0.19, and SPIONs encapsulation efficiency (%EE) around 76%. Cytocompatibility assays showed that no toxicity was observed in fibroblasts, for iron concentrations up to 400μg/ml. Also, for safe lipid and iron concentrations, no hemolytic effect was detected. The fusogenicity of the nanosystems was first evaluated through lipid mixing assays, based on Förster resonance energy transfer (FRET), using liposomal membrane models, mimicking bacterial cytoplasmic membrane and eukaryotic plasma membrane. It was shown that the hybrid nanosystems preferentially interact with the bacterial membrane model. Confocal microscopy and fluorescence lifetime measurements, using giant unilamellar vesicles (GUVs), validated these results. Overall, the developed hybrid nanosystem may represent an efficient drug delivery system with improved targetability for bacterial membrane.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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