Effect of whole oilseeds in the diet on bacterial diversity in the solid fraction of the ruminal content of steers.

Autor: de Melo HSA; College of Veterinary Medicine and Animal Science, Federal University of Mato Grosso do Sul-UFMS, Campo Grande, MS, Brazil., Ítavo LCV; College of Veterinary Medicine and Animal Science, Federal University of Mato Grosso do Sul-UFMS, Campo Grande, MS, Brazil. luis.itavo@ufms.br.; Faculdade de Medicina Veterinária e Zootecnia-FAMEZ, Universidade Federal de Mato Grosso do Sul - UFMS, Av. Senador Filinto Müller, 2443, Cidade Universitária, Campo Grande, MS, 79070-900, Brazil. luis.itavo@ufms.br., de Castro AP; Department of Biotechnology, Dom Bosco Catholic University, Campo Grande, MS, Brazil., Ítavo CCBF; College of Veterinary Medicine and Animal Science, Federal University of Mato Grosso do Sul-UFMS, Campo Grande, MS, Brazil., de Araújo Caldas R; College of Veterinary Medicine and Animal Science, Federal University of Mato Grosso do Sul-UFMS, Campo Grande, MS, Brazil., Mateus RG; Department of Biotechnology, Dom Bosco Catholic University, Campo Grande, MS, Brazil., Niwa MVG; College of Veterinary Medicine and Animal Science, Federal University of Mato Grosso do Sul-UFMS, Campo Grande, MS, Brazil., de Moraes GJ; College of Veterinary Medicine and Animal Science, Federal University of Mato Grosso do Sul-UFMS, Campo Grande, MS, Brazil., da Silva Zornitta C; College of Veterinary Medicine and Animal Science, Federal University of Mato Grosso do Sul-UFMS, Campo Grande, MS, Brazil., Gurgel ALC; Department of Animal Science, State University of Maringá, Maringá, PR, Brazil., Benchaar C; Sherbrooke Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, QC, Canada.
Jazyk: angličtina
Zdroj: Tropical animal health and production [Trop Anim Health Prod] 2023 Jan 05; Vol. 55 (1), pp. 32. Date of Electronic Publication: 2023 Jan 05.
DOI: 10.1007/s11250-022-03442-x
Abstrakt: Our hypothesis was that different whole oilseeds included in the diet for steers confined could alter the diversity of rumen bacteria compared to a diet without oilseeds or an exclusively forage diet. It was aimed to evaluate the effects of oilseeds inclusion in the diet on bacterial diversity in the solid fraction of the ruminal content of steers, by gene sequences of the conserved 16S rDNA region. Six crossbred steers castrated males, fitted with ruminal cannula were used in a 6 × 6 Latin square design, using 21-day period. At the start of the experiment, the live weight of the animals averaged 416 ± 9.7 kg (mean ± SD). A total of 2,180,562 16S rDNA sequences were generated for the Bacteria domain by MiSeq sequencing. The bacterial diversity was composed of 24 bacterial phyla, with the most abundant being Firmicutes, Bacteroidetes, and Proteobacteria. Other phyla with less diversity were also identified including Eurychaeota, Tenericutes, SR1 Absconditalbacteria, Synergistetes, Actinobacteria, Saccharibacteria, Elusimicrobia, Cyanobacteria, Verrucomicrobia, Fusobacteria, Lentisphaerae. The similarity in the bacterial community averaged 50% for all the experimental diets. Steers-fed corn silage exhibited a great diversity of bacteria of the Firmicutes phylum. The steers-fed oilseeds in the diet had a great diversity of bacteria from the phylum Bacteroidetes and Proteobacteria. The inclusion of whole oilseeds in the steer diets can alter the rumen bacteria population by up to 50% of total diversity.
(© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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