Characterization of a Lipopolysaccharide- and Beta-1,3-Glucan Binding Protein (LGBP) from the Hepatopancreas of Freshwater Prawn, Macrobrachium rosenbergii, Possessing Lectin-Like Activity.

Autor: Sahoo S; ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, India., Badhe MR; ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, India., Paul A; ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, India., Sahoo PK; ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, India., Suryawanshi AR; Institute of Life Sciences, Bhubaneswar, 751023, India., Panda D; ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, India., Pillai BR; ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, India., Patnaik BB; P.G. Department of Biosciences and Biotechnology, Fakir Mohan University, Vyasa Vihar, Nuapadhi, Balasore, 756089, India.; Department of Biology, College of Natural Sciences, Soonchunhyang University, Asan, Chungcheongnam-do, 31538, Korea., Mohanty J; ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, India. jmohantycifa@gmail.com.
Jazyk: angličtina
Zdroj: Probiotics and antimicrobial proteins [Probiotics Antimicrob Proteins] 2023 Dec; Vol. 15 (6), pp. 1596-1607. Date of Electronic Publication: 2023 Jan 03.
DOI: 10.1007/s12602-022-10021-x
Abstrakt: The study focuses on the isolation, characterization, and expression analysis of a lectin from the hepatopancreas of Macrobrachium rosenbergii. The protein was isolated by affinity chromatography on a melibiose-agarose column. The molecular weight of the native protein was found to be ~120 kDa which consists of a single polypeptide of ~39.5 kDa. On mass spectrometric analysis, the protein was identified as lipopolysaccharide- and beta-1,3-glucan binding protein (LGBP). LGBP showed hemagglutination with rabbit RBC like a lectin and its carbohydrate-binding specificity was determined by the hemagglutination inhibition test. The protein also showed antibacterial activity against two Gram-negative bacteria Vibrio harveyi and Aeromonas sobria, and one Gram positive bacteria Bacillus cereus in the disc diffusion test. Rabbit antiserum was raised against the purified LGBP and used to develop a sandwich ELISA system for quantitation of the protein in hepatopancreas and serum samples of M. rosenbergii. The expression of the LGBP transcripts in muscle, hepatopancreas, and gill tissues from M. rosenbergii juveniles at 72 h post-challenge of V. harveyi was not modulated as noticed in qPCR analysis. However, significant increases in the concentrations of LGBP protein in hepatopancreas (5.23 ± 0.45 against 3.43 ± 0.43 mg/g tissue in control) and serum (1.08 ± 0.14 against 0.61 ± 0.08 µg/ml in control) were observed in the challenged group of prawns in ELISA suggesting its putative role against bacterial infections. The study for the first time characterized the native LGBP of M. rosenbergii showing a multifunctional role in immunity.
(© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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