Electrochemical Capillary Driven Immunoassay for Detection of SARS-CoV-2.

Autor:	Clark KM; Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523, United States., Schenkel MS; Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523, United States., Pittman TW; Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523, United States., Samper IC; Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523, United States.; Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, Colorado 80523, United States., Anderson LBR; Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523, United States., Khamcharoen W; Department of Chemistry, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand., Elmegerhi S; Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523, United States., Perera R; Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523, United States., Siangproh W; Department of Chemistry, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand., Kennan AJ; Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523, United States., Geiss BJ; Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523, United States.; School of Biomedical Engineering, Colorado State University, Fort Collins, Colorado 80523, United States., Dandy DS; Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, Colorado 80523, United States.; School of Biomedical Engineering, Colorado State University, Fort Collins, Colorado 80523, United States., Henry CS; Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523, United States.; Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, Colorado 80523, United States.; School of Biomedical Engineering, Colorado State University, Fort Collins, Colorado 80523, United States.
Jazyk:	angličtina
Zdroj:	ACS measurement science au [ACS Meas Sci Au] 2022 Dec 21; Vol. 2 (6), pp. 584-594. Date of Electronic Publication: 2022 Aug 30.
DOI:	10.1021/acsmeasuresciau.2c00037
Abstrakt:	The COVID-19 pandemic focused attention on a pressing need for fast, accurate, and low-cost diagnostic tests. This work presents an electrochemical capillary driven immunoassay (eCaDI) developed to detect SARS-CoV-2 nucleocapsid (N) protein. The low-cost flow device is made of polyethylene terephthalate (PET) and adhesive films. Upon addition of a sample, reagents and washes are sequentially delivered to an integrated screen-printed carbon electrode for detection, thus automating a full sandwich immunoassay with a single end-user step. The modified electrodes are sensitive and selective for SARS-CoV-2 N protein and stable for over 7 weeks. The eCaDI was tested with influenza A and Sindbis virus and proved to be selective. The eCaDI was also successfully applied to detect nine different SARS-CoV-2 variants, including Omicron. Competing Interests: The authors declare no competing financial interest. (© 2022 The Authors. Published by American Chemical Society.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu