Dendritic Cell Membrane-Derived Nanovesicles for Targeted T Cell Activation.

Autor:	Harvey BT; Department of Chemistry, College of Arts and Sciences, University of Kentucky, Lexington, Kentucky 40506, United States., Fu X; Light Microscopy Facility, University of Kentucky, Lexington, Kentucky 40506, United States., Li L; Department of Chemistry, College of Arts and Sciences, University of Kentucky, Lexington, Kentucky 40506, United States., Neupane KR; Department of Chemistry, College of Arts and Sciences, University of Kentucky, Lexington, Kentucky 40506, United States., Anand N; Department of Pharmacy and Practice, College of Pharmacy, University of Kentucky, Lexington, Kentucky 40506, United States., Kolesar JM; Department of Pharmacy and Practice, College of Pharmacy, University of Kentucky, Lexington, Kentucky 40506, United States., Richards CI; Department of Chemistry, College of Arts and Sciences, University of Kentucky, Lexington, Kentucky 40506, United States.
Jazyk:	angličtina
Zdroj:	ACS omega [ACS Omega] 2022 Dec 09; Vol. 7 (50), pp. 46222-46233. Date of Electronic Publication: 2022 Dec 09 (Print Publication: 2022).
DOI:	10.1021/acsomega.2c04420
Abstrakt:	T cells play an integral role in the generation of an effective immune response and are responsible for clearing foreign microbes that have bypassed innate immune system defenses and possess cognate antigens. The immune response can be directed toward a desired target through the selective priming and activation of T cells. Due to their ability to activate a T cell response, dendritic cells and endogenous vesicles from dendritic cells are being developed for cancer immunotherapy treatment. However, current platforms, such as exosomes and synthetic nanoparticles, are limited by their production methods and application constraints. Here, we engineer nanovesicles derived from dendritic cell membranes with similar properties as dendritic cell exosomes via nitrogen cavitation. These cell-derived nanovesicles are capable of activating antigen-specific T cells through direct and indirect mechanisms. Additionally, these nanovesicles can be produced in large yields, overcoming production constraints that limit clinical application of alternative immunomodulatory vesicle or nanoparticle-based methods. Thus, dendritic cell-derived nanovesicles generated by nitrogen cavitation show potential as an immunotherapy platform to stimulate and direct T cell response. Competing Interests: The authors declare no competing financial interest. (© 2022 The Authors. Published by American Chemical Society.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu