Evaluation of engineered low-lignin poplar for conversion into advanced bioproducts.

Autor: Lin CY; DOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA.; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Geiselman GM; DOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA.; Department of Biomaterials and Biomanufacturing, Sandia National Laboratories, Livermore, CA, 94550, USA.; DOE, Agile BioFoundry, Emeryville, CA, 94608, USA., Liu D; Department of Biomaterials and Biomanufacturing, Sandia National Laboratories, Livermore, CA, 94550, USA.; DOE, Agile BioFoundry, Emeryville, CA, 94608, USA., Magurudeniya HD; DOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA.; Department of Biomaterials and Biomanufacturing, Sandia National Laboratories, Livermore, CA, 94550, USA., Rodriguez A; DOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA.; Department of Biomaterials and Biomanufacturing, Sandia National Laboratories, Livermore, CA, 94550, USA.; DOE, Agile BioFoundry, Emeryville, CA, 94608, USA., Chen YC; DOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA.; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Pidatala V; DOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA.; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Unda F; Department of Wood Science, University of British Columbia, Vancouver, BC, Canada., Amer B; DOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA.; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Baidoo EEK; DOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA.; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Mansfield SD; Department of Wood Science, University of British Columbia, Vancouver, BC, Canada.; DOE Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, Madison, WI, 53726, USA., Simmons BA; DOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA.; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Singh S; DOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA.; Department of Bioresources and Environmental Security, Sandia National Laboratories, Livermore, CA, 94550, USA., Scheller HV; DOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA.; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Plant and Microbial Biology, University of California, Berkeley, CA, 94720, USA., Gladden JM; DOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA. JMGladden@lbl.gov.; Department of Biomaterials and Biomanufacturing, Sandia National Laboratories, Livermore, CA, 94550, USA. JMGladden@lbl.gov.; DOE, Agile BioFoundry, Emeryville, CA, 94608, USA. JMGladden@lbl.gov., Eudes A; DOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA. AGEudes@lbl.gov.; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA. AGEudes@lbl.gov.
Jazyk: angličtina
Zdroj: Biotechnology for biofuels and bioproducts [Biotechnol Biofuels Bioprod] 2022 Dec 25; Vol. 15 (1), pp. 145. Date of Electronic Publication: 2022 Dec 25.
DOI: 10.1186/s13068-022-02245-4
Abstrakt: Background: Lignocellulosic resources are promising feedstocks for the manufacture of bio-based products and bioenergy. However, the inherent recalcitrance of biomass to conversion into simple sugars currently hinders the deployment of advanced bioproducts at large scale. Lignin is a primary contributor to biomass recalcitrance as it protects cell wall polysaccharides from degradation and can inhibit hydrolytic enzymes via non-productive adsorption. Several engineering strategies have been designed to reduce lignin or modify its monomeric composition. For example, expression of bacterial 3-dehydroshikimate dehydratase (QsuB) in poplar trees resulted in a reduction in lignin due to redirection of metabolic flux toward 3,4-dihydroxybenzoate at the expense of lignin. This reduction was accompanied with remarkable changes in the pools of aromatic compounds that accumulate in the biomass.
Results: The impact of these modifications on downstream biomass deconstruction and conversion into advanced bioproducts was evaluated in the current study. Using ionic liquid pretreatment followed by enzymatic saccharification, biomass from engineered trees released more glucose and xylose compared to wild-type control trees under optimum conditions. Fermentation of the resulting hydrolysates using Rhodosporidium toruloides strains engineered to produce α-bisabolene, epi-isozizaene, and fatty alcohols showed no negative impact on cell growth and yielded higher titers of bioproducts (as much as + 58%) in the case of QsuB transgenics trees.
Conclusion: Our data show that low-recalcitrant poplar biomass obtained with the QsuB technology has the potential to improve the production of advanced bioproducts.
(© 2022. The Author(s).)
Databáze: MEDLINE
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