Cytotoxic and mutagenic effects of the food additive tartrazine on eukaryotic cells.

Autor: Dos Santos JR; Laboratory of Cytogenetics and Mutagenesis of the Federal University of Piauí, Picos, Brazil., de Sousa Soares L; Laboratory of Cytogenetics and Mutagenesis of the Federal University of Piauí, Picos, Brazil., Soares BM; Laboratory of Human Cytogenetics and Oncology Research Center, Federal University of Pará, Belém, Brazil., de Gomes Farias M; Laboratory of Cytogenetics and Mutagenesis of the Federal University of Piauí, Picos, Brazil., de Oliveira VA; Laboratory of Cytogenetics and Mutagenesis of the Federal University of Piauí, Picos, Brazil., de Sousa NAB; Laboratory of Cytogenetics and Mutagenesis of the Federal University of Piauí, Picos, Brazil., Negreiros HA; Laboratory of Cytogenetics and Mutagenesis of the Federal University of Piauí, Picos, Brazil., da Silva FCC; Laboratory of Cytogenetics and Mutagenesis of the Federal University of Piauí, Picos, Brazil.; Cytogenetic and Mutagenesis Laboratory, Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, Brazil., Peron AP; Laboratory of Cytogenetics and Mutagenesis of the Federal University of Piauí, Picos, Brazil.; Cytogenetic and Mutagenesis Laboratory, Postgraduate Program in Genetics and Improvement of the Federal University of Piauí, Teresina, Brazil., Pacheco ACL; Laboratory of Parasitology and ecology of neglected diseases, Federal University of Piaui, Terezina, Brazil., Marques MMM; Laboratory of Parasitology and ecology of neglected diseases, Federal University of Piaui, Terezina, Brazil., Gonçalves JCR; Department of Biochemistry and Pharmacology, Federal University of Piaui, Terezina, Brazil., Montenegro RC; Drug Development and Research Center, Federal University of Ceara, Fortaleza, Brazil., Islam MT; Department of Pharmacy, Life Science Faculty, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj8100, Dhaka, Bangladesh. dmt.islam@bsmrstu.edu.bd., Sharifi-Rad J; Facultad de Medicina, Universidad del Azuay, Cuenca, Ecuador. Javad.sharifirad@gmail.com., Mubarak MS; Department of Chemistry, The University of Jordan, Amman, 11942, Jordan., de Melo Cavalcante AAC; Cytogenetic and Mutagenesis Laboratory, Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, Brazil., de Castro E Sousa JM; Laboratory of Cytogenetics and Mutagenesis of the Federal University of Piauí, Picos, Brazil.; Cytogenetic and Mutagenesis Laboratory, Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, Brazil.
Jazyk: angličtina
Zdroj: BMC pharmacology & toxicology [BMC Pharmacol Toxicol] 2022 Dec 23; Vol. 23 (1), pp. 95. Date of Electronic Publication: 2022 Dec 23.
DOI: 10.1186/s40360-022-00638-7
Abstrakt: Background: Among the food additives used in the food industry, food dyes are considered the most toxic. For instance, tartrazine (TRZ) is a food colorant commercially available with conflicting data regarding its cytotoxic, genotoxic, and mutagenic effects. Therefore, this study aimed to evaluate the cytotoxic and mutagenic potential of TRZ using different eukaryotic cells (in vitro).
Methods: This study employed 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), brine shrimp lethality, Allium cepa and Saccharomyces cerevisiae tests. Different concentrations of TRZ and different exposure times were used in this study.
Results: The results demonstrate that TRZ induced a concentration-dependent toxic effect on the test systems. It also exerted cytotoxicity in fibroblasts and human gastric cells. In addition, TRZ showed mutagenic effects on the A. cepa test system. However, its toxicogenic effects may not relate to the oxidizing activity, which was confirmed by the S. cerevisiae test model.
Conclusion: Taken together, TRZ exerted toxicogenic effects on the test systems. Therefore, it may be harmful to health, especially its prolonged use may trigger carcinogenesis.
(© 2022. The Author(s).)
Databáze: MEDLINE
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