Engineering Nicotiana tabacum trichomes for triterpenic acid production.

Autor: Gossart N; Louvain Institute of Biomolecular Science and Technology, UCLouvain, Louvain-la-Neuve, Belgium., Berhin A; Louvain Institute of Biomolecular Science and Technology, UCLouvain, Louvain-la-Neuve, Belgium., Sergeant K; Environmental Research and Innovation, Luxembourg Institute of Science and Technology, Esch-sur-Alzette, Luxembourg., Alam I; Louvain Institute of Biomolecular Science and Technology, UCLouvain, Louvain-la-Neuve, Belgium; Plant Biotechnology Division, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka 1349, Bangladesh., André C; Environmental Research and Innovation, Luxembourg Institute of Science and Technology, Esch-sur-Alzette, Luxembourg; The New Zealand Institute for Plant and Food Research Ltd (PFR), Private Bag 92169, Auckland, New Zealand., Hausman JF; Environmental Research and Innovation, Luxembourg Institute of Science and Technology, Esch-sur-Alzette, Luxembourg., Boutry M; Louvain Institute of Biomolecular Science and Technology, UCLouvain, Louvain-la-Neuve, Belgium., Hachez C; Louvain Institute of Biomolecular Science and Technology, UCLouvain, Louvain-la-Neuve, Belgium. Electronic address: charles.hachez@uclouvain.be.
Jazyk: angličtina
Zdroj: Plant science : an international journal of experimental plant biology [Plant Sci] 2023 Mar; Vol. 328, pp. 111573. Date of Electronic Publication: 2022 Dec 21.
DOI: 10.1016/j.plantsci.2022.111573
Abstrakt: In this work, we aimed at implementing the biosynthesis of triterpenic acids in Nicotiana tabacum glandular trichomes. Although endogenous genes coding for enzymes involved in such biosynthetic pathway are found in the Nicotiana tabacum genome, implementing such pathway specifically in glandular trichomes required to boost endogenous enzymatic activities. Five transgenes coding for a farnesyl-diphosphate synthase, a squalene synthase, a squalene epoxidase, a beta-amyrin synthase and a beta-amyrin 28-monooxygenase were introduced in N.tabacum, their expression being driven by pMALD1, a trichome-specific transcriptional promoter. This study aimed at testing whether sinking isoprenoid precursors localized in plastids, by exploiting potential cross-talks allowing the exchange of terpenoid pools from the chloroplast to the cytosol, could be a way to improve overall yield. By analyzing metabolites extracted from entire leaves, a low amount of ursolic acid was detected in plants expressing the five transgenes. Our study shows that the terpene biosynthetic pathway could be, in part, redirected in N.tabacum glandular trichomes with no deleterious phenotype at the whole plant level (chlorosis, dwarfism,…). In light of our results, possible ways to improve the final yield are discussed.
Competing Interests: Conflict of interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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