Guidelines for mouse and human DC functional assays.

Autor: Clausen BE; Research Center for Immunotherapy (FZI), University Medical Center of the Johannes-Gutenberg University Mainz, Mainz, Germany.; Institute for Molecular Medicine, Paul Klein Center for Immune Intervention, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany., Amon L; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Germany., Backer RA; Research Center for Immunotherapy (FZI), University Medical Center of the Johannes-Gutenberg University Mainz, Mainz, Germany.; Institute for Molecular Medicine, Paul Klein Center for Immune Intervention, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany., Berod L; Research Center for Immunotherapy (FZI), University Medical Center of the Johannes-Gutenberg University Mainz, Mainz, Germany.; Institute of Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Germany., Bopp T; Research Center for Immunotherapy (FZI), University Medical Center of the Johannes-Gutenberg University Mainz, Mainz, Germany.; Institute of Immunology, Paul Klein Center for Immune Intervention, University Medical Center of the Johannes-Gutenberg University Mainz, Mainz, Germany., Brand A; Institute for Molecular Medicine, Paul Klein Center for Immune Intervention, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany., Burgdorf S; Laboratory of Cellular Immunology, LIMES Institute, University of Bonn, Bonn, Germany., Chen L; Department of Dermatology, University Hospital Heidelberg, Heidelberg, Germany., Da M; Department of Dermatology, University Hospital Heidelberg, Heidelberg, Germany., Distler U; Research Center for Immunotherapy (FZI), University Medical Center of the Johannes-Gutenberg University Mainz, Mainz, Germany.; Institute of Immunology, Paul Klein Center for Immune Intervention, University Medical Center of the Johannes-Gutenberg University Mainz, Mainz, Germany., Dress RJ; Institute of Systems Immunology, Hamburg Center for Translational Immunology (HCTI), University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Dudziak D; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Germany.; Medical Immunology Campus Erlangen (MICE), Erlangen, Germany.; Deutsches Zentrum Immuntherapie (DZI), Germany., Dutertre CA; Gustave Roussy Cancer Campus, Villejuif, France.; Institut National de la Santé et de la Recherche Médicale (INSERM) U1015, Equipe Labellisée-Ligue Nationale contre le Cancer, Villejuif, France., Eich C; Institute for Molecular Medicine, Paul Klein Center for Immune Intervention, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany., Gabele A; Research Center for Immunotherapy (FZI), University Medical Center of the Johannes-Gutenberg University Mainz, Mainz, Germany.; Institute of Immunology, Paul Klein Center for Immune Intervention, University Medical Center of the Johannes-Gutenberg University Mainz, Mainz, Germany., Geiger M; Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University, Medical Faculty, Aachen, Germany.; Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany., Ginhoux F; Gustave Roussy Cancer Campus, Villejuif, France.; Institut National de la Santé et de la Recherche Médicale (INSERM) U1015, Equipe Labellisée-Ligue Nationale contre le Cancer, Villejuif, France.; Singapore Immunology Network (SIgN), Agency for Science, Technology and Research, Singapore, Singapore.; Shanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.; Translational Immunology Institute, SingHealth Duke-NUS Academic Medical Centre, Singapore, Singapore., Giusiano L; Institute of Medical Microbiology and Hygiene, University Medical Center of the Johannes Gutenberg-University Mainz, Germany., Godoy GJ; Institute of Medical Microbiology and Hygiene, University Medical Center of the Johannes Gutenberg-University Mainz, Germany., Hamouda AEI; Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University, Medical Faculty, Aachen, Germany.; Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany., Hatscher L; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Germany., Heger L; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Germany., Heidkamp GF; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Germany., Hernandez LC; Cell Communication and Migration Laboratory, Institute of Biochemistry and Molecular Cell Biology, Center for Experimental Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Jacobi L; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Germany., Kaszubowski T; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Germany., Kong WT; Gustave Roussy Cancer Campus, Villejuif, France.; Institut National de la Santé et de la Recherche Médicale (INSERM) U1015, Equipe Labellisée-Ligue Nationale contre le Cancer, Villejuif, France., Lehmann CHK; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Germany.; Medical Immunology Campus Erlangen (MICE), Erlangen, Germany.; Deutsches Zentrum Immuntherapie (DZI), Germany., López-López T; Cell Communication and Migration Laboratory, Institute of Biochemistry and Molecular Cell Biology, Center for Experimental Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Mahnke K; Department of Dermatology, University Hospital Heidelberg, Heidelberg, Germany., Nitsche D; Laboratory of Cellular Immunology, LIMES Institute, University of Bonn, Bonn, Germany., Renkawitz J; Biomedical Center (BMC), Walter Brendel Center of Experimental Medicine, Institute of Cardiovascular Physiology and Pathophysiology, Klinikum der Universität, LMU Munich, Munich, Germany., Reza RA; Biomedical Center (BMC), Walter Brendel Center of Experimental Medicine, Institute of Cardiovascular Physiology and Pathophysiology, Klinikum der Universität, LMU Munich, Munich, Germany., Sáez PJ; Cell Communication and Migration Laboratory, Institute of Biochemistry and Molecular Cell Biology, Center for Experimental Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Schlautmann L; Laboratory of Cellular Immunology, LIMES Institute, University of Bonn, Bonn, Germany., Schmitt MT; Biomedical Center (BMC), Walter Brendel Center of Experimental Medicine, Institute of Cardiovascular Physiology and Pathophysiology, Klinikum der Universität, LMU Munich, Munich, Germany., Seichter A; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Germany., Sielaff M; Research Center for Immunotherapy (FZI), University Medical Center of the Johannes-Gutenberg University Mainz, Mainz, Germany.; Institute of Immunology, Paul Klein Center for Immune Intervention, University Medical Center of the Johannes-Gutenberg University Mainz, Mainz, Germany., Sparwasser T; Research Center for Immunotherapy (FZI), University Medical Center of the Johannes-Gutenberg University Mainz, Mainz, Germany.; Institute of Medical Microbiology and Hygiene, University Medical Center of the Johannes Gutenberg-University Mainz, Germany., Stoitzner P; Department of Dermatology, Venerology & Allergology, Medical University Innsbruck, Innsbruck, Austria., Tchitashvili G; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Germany., Tenzer S; Research Center for Immunotherapy (FZI), University Medical Center of the Johannes-Gutenberg University Mainz, Mainz, Germany.; Institute of Immunology, Paul Klein Center for Immune Intervention, University Medical Center of the Johannes-Gutenberg University Mainz, Mainz, Germany.; Helmholtz Institute for Translational Oncology Mainz (HI-TRON Mainz), Mainz, Germany., Tochoedo NR; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Germany., Vurnek D; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Germany., Zink F; Laboratory of Cellular Immunology, LIMES Institute, University of Bonn, Bonn, Germany., Hieronymus T; Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University, Medical Faculty, Aachen, Germany.; Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany.; Institute of Cell and Tumor Biology, RWTH Aachen University, Medical Faculty, Germany.
Jazyk: angličtina
Zdroj: European journal of immunology [Eur J Immunol] 2023 Dec; Vol. 53 (12), pp. e2249925. Date of Electronic Publication: 2022 Dec 23.
DOI: 10.1002/eji.202249925
Abstrakt: This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs and various non-lymphoid tissues. Recent studies have provided evidence for an increasing number of phenotypically distinct conventional DC (cDC) subsets that on one hand exhibit a certain functional plasticity, but on the other hand are characterized by their tissue- and context-dependent functional specialization. Here, we describe a selection of assays for the functional characterization of mouse and human cDC. The first two protocols illustrate analysis of cDC endocytosis and metabolism, followed by guidelines for transcriptomic and proteomic characterization of cDC populations. Then, a larger group of assays describes the characterization of cDC migration in vitro, ex vivo, and in vivo. The final guidelines measure cDC inflammasome and antigen (cross)-presentation activity. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all co-authors, making it an essential resource for basic and clinical DC immunologists.
(© 2022 The Authors. European Journal of Immunology published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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