Antioxidant and Chemopreventive Activity of Protein Hydrolysates from Raw and Germinated Flour of Legumes with Commercial Interest in Colorectal Cancer.

Autor:	Fuel M; Institute of Biopathology and Regenerative Medicine (IBIMER), Center of Biomedical Research (CIBM), University of Granada, 18100 Granada, Spain., Mesas C; Institute of Biopathology and Regenerative Medicine (IBIMER), Center of Biomedical Research (CIBM), University of Granada, 18100 Granada, Spain.; Department of Anatomy and Embryology, Faculty of Medicine, University of Granada, 18071 Granada, Spain.; Instituto Biosanitario de Granada (ibs.GRANADA), 18014 Granada, Spain., Martínez R; Cellbitec S.L., N.I.F. B04847216, Scientific Headquarters of the Almería Technology Park, Universidad de Almería, 04128 La Cañada, Spain.; Department of Physiology, Institute of Nutrition and Food Technology (INyTA), Biomedical Research Center (CIBM), Universidad de Granada, 18100 Granada, Spain., Ortiz R; Institute of Biopathology and Regenerative Medicine (IBIMER), Center of Biomedical Research (CIBM), University of Granada, 18100 Granada, Spain.; Department of Anatomy and Embryology, Faculty of Medicine, University of Granada, 18071 Granada, Spain.; Instituto Biosanitario de Granada (ibs.GRANADA), 18014 Granada, Spain., Quiñonero F; Institute of Biopathology and Regenerative Medicine (IBIMER), Center of Biomedical Research (CIBM), University of Granada, 18100 Granada, Spain.; Department of Anatomy and Embryology, Faculty of Medicine, University of Granada, 18071 Granada, Spain.; Instituto Biosanitario de Granada (ibs.GRANADA), 18014 Granada, Spain., Bermúdez F; Cellbitec S.L., N.I.F. B04847216, Scientific Headquarters of the Almería Technology Park, Universidad de Almería, 04128 La Cañada, Spain., Gutiérrez N; IFAPA Centro Alameda del Obispo, Área de Genómica y Biotecnología, Apdo 3092, 14080 Córdoba, Spain., Torres AM; IFAPA Centro Alameda del Obispo, Área de Genómica y Biotecnología, Apdo 3092, 14080 Córdoba, Spain., Kapravelou G; Department of Physiology, Institute of Nutrition and Food Technology (INyTA), Biomedical Research Center (CIBM), Universidad de Granada, 18100 Granada, Spain., Lozano A; Department of Physiology, Institute of Nutrition and Food Technology (INyTA), Biomedical Research Center (CIBM), Universidad de Granada, 18100 Granada, Spain., Perazzoli G; Institute of Biopathology and Regenerative Medicine (IBIMER), Center of Biomedical Research (CIBM), University of Granada, 18100 Granada, Spain.; Instituto Biosanitario de Granada (ibs.GRANADA), 18014 Granada, Spain., Prados J; Institute of Biopathology and Regenerative Medicine (IBIMER), Center of Biomedical Research (CIBM), University of Granada, 18100 Granada, Spain.; Department of Anatomy and Embryology, Faculty of Medicine, University of Granada, 18071 Granada, Spain.; Instituto Biosanitario de Granada (ibs.GRANADA), 18014 Granada, Spain., Porres JM; Department of Physiology, Institute of Nutrition and Food Technology (INyTA), Biomedical Research Center (CIBM), Universidad de Granada, 18100 Granada, Spain., Melguizo C; Institute of Biopathology and Regenerative Medicine (IBIMER), Center of Biomedical Research (CIBM), University of Granada, 18100 Granada, Spain.; Department of Anatomy and Embryology, Faculty of Medicine, University of Granada, 18071 Granada, Spain.; Instituto Biosanitario de Granada (ibs.GRANADA), 18014 Granada, Spain.
Jazyk:	angličtina
Zdroj:	Antioxidants (Basel, Switzerland) [Antioxidants (Basel)] 2022 Dec 08; Vol. 11 (12). Date of Electronic Publication: 2022 Dec 08.
DOI:	10.3390/antiox11122421
Abstrakt:	Legumes are a highly nutritious source of plant protein, fiber, minerals and vitamins. However, they also contain several bioactive compounds with significant potential benefits for human health. The objectives of this study were to evaluate the antioxidant, antitumor and chemopreventive activity of functional extracts from legumes using raw and germinated flours of six legume species of commercial interest. The methodology carried out consisted on the development of protein hydrolysates, assessment of their antioxidant capacity and in vitro tests on T84, HCT15 and SW480 colorectal cancer (CRC) cell lines. Our results showed a high antitumor activity of protein hydrolysate from M. sativa . Likewise, when combined with 5-Fluorouracile (5-Fu), there was a synergistic effect using extract concentrations from 50 to 175 µg/mL and 5-Fu concentrations from 1.5 to 5 µM. Similarly, the induction effect on detoxifying enzymes by the extracts of M. sativa , germinated V. faba Baraca × LVzt1 and V. narbonensis , which produced a higher induction rate than the positive control sulforaphane (10 µM), should be highlighted. Therefore, incorporating these enzymes into the diet could provide nutritional effects, as well as play an effective role in cancer chemoprevention and therapy.
Databáze:	MEDLINE
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