Oligopeptide Sortase Inhibitor Modulates Staphylococcus aureus Cell Adhesion and Biofilm Formation.

Autor:	Bozhkova SA; Vreden National Medical Research Center of Traumatology and Orthopedics, 195427 St. Petersburg, Russia., Gordina EM; Vreden National Medical Research Center of Traumatology and Orthopedics, 195427 St. Petersburg, Russia., Labutin DV; Vreden National Medical Research Center of Traumatology and Orthopedics, 195427 St. Petersburg, Russia., Kudryavtsev KV; Laboratory of Molecular Pharmacology, Pirogov Russian National Research Medical University, 117997 Moscow, Russia.
Jazyk:	angličtina
Zdroj:	Antibiotics (Basel, Switzerland) [Antibiotics (Basel)] 2022 Dec 17; Vol. 11 (12). Date of Electronic Publication: 2022 Dec 17.
DOI:	10.3390/antibiotics11121836
Abstrakt:	Prevention of bacterial adhesion is one of the most important antivirulence strategies for meeting the global challenge posed by antimicrobial resistance. We aimed to investigate the influence of a peptidic S. aureus sortase A inhibitor on bacterial adhesion to eukaryotic cells and biofilm formation as a potential method for reducing S. aureus virulence. The pentapeptide LPRDA was synthesized and characterized as a pure individual organic compound. Incubation of MSSA and MRSA strains with LPRDA induced a subsequent reduction in staphylococcal adhesion to Vero cells and biofilm formation, as visualized by microscopic and spectrophotometric methods, respectively. LPRDA did not have a cytotoxic effect on eukaryotic or bacterial cells. The pentapeptide LPRDA deserves further investigation using in vitro and in vivo models of Gram-positive bacteriemia as a potential antibacterial agent with an antiadhesive mechanism of action. Competing Interests: The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of the data; in the writing of the manuscript; or in the decision to publish the results.
Databáze:	MEDLINE
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