Topical delivery of dsRNA in two hemipteran species: Evaluation of RNAi specificity and non-target effects.

Autor: Finetti L; Department of Biology, University of Toronto Mississauga, Mississauga, ON, Canada. Electronic address: luca.finetti@utoronto.ca., Benetti L; Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy., Leyria J; Department of Biology, University of Toronto Mississauga, Mississauga, ON, Canada., Civolani S; Department of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, Ferrara, Italy., Bernacchia G; Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.
Jazyk: angličtina
Zdroj: Pesticide biochemistry and physiology [Pestic Biochem Physiol] 2023 Jan; Vol. 189, pp. 105295. Date of Electronic Publication: 2022 Nov 19.
DOI: 10.1016/j.pestbp.2022.105295
Abstrakt: Double-stranded (ds) RNA-based technologies could provide novel and potential tool for pest management with efficiency and specificity of action. However, before applying this technique in the field, it is necessary to identify effective delivery methods and evaluate the non-target effects that may occur. In this article, we evaluated the effectiveness of dsRNA by topical delivery on a species of great agricultural interest, Halyomorpha halys. The specificity of action of the dsRNA was also investigated in Rhodnius prolixus, an insect phylogenetically close to H. halys. Of the three investigated genes (putative ATPase N2B, ATPase, serine/threonine-protein phosphatase PP1-β catalytic subunit, PP1, and IAP repeat-containing protein 7-B-like, IAP), IAP and ATPase were able to induce higher mortality in H. halys nymphs compared to the control, with specific concentrations for each gene targeted. However, when the same RNAs were topically delivered to both R. prolixus 2nd and 3rd instar nymphs, no gene silencing and mortality were observed. For this reason, to assess dsRNA application-mediated non-target effects, we injected both H. halys and R. prolixus specific dsRNA in R. prolixus 5th instar nymphs. When the dsRNA targeting H. halys IAP was microinjected into R. prolixus 5th instar nymphs, no mortality was observed, suggesting a strong RNAi specificity. Together, these data suggest that the topical delivery could be suitable for the dsRNA to control H. halys population. Furthermore, its specificity of action would allow treatments towards single harmful species with limited non-target effects.
Competing Interests: Declaration of Competing Interest The authors declare no potential conflict of interest.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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