Adaptive immune responses to SARS-CoV-2 persist in the pharyngeal lymphoid tissue of children.

Autor:	Xu Q; Cell Signaling and Immunity Section, Laboratory of Immune System Biology (LISB), National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD, USA., Milanez-Almeida P; Center for Human Immunology, NIAID, NIH, Bethesda, MD, USA., Martins AJ; Multiscale Systems Biology Section, LISB, NIAID, NIH, Bethesda, MD, USA., Radtke AJ; Center for Advanced Tissue Imaging, LISB, NIAID, NIH, Bethesda, MD, USA., Hoehn KB; Department of Pathology, Yale School of Medicine, New Haven, CT, USA., Oguz C; NIAID Collaborative Bioinformatics Resource (NCBR), NIAID, NIH, Bethesda, MD, USA.; Axle Informatics, Bethesda, MD, USA., Chen J; Center for Human Immunology, NIAID, NIH, Bethesda, MD, USA., Liu C; Multiscale Systems Biology Section, LISB, NIAID, NIH, Bethesda, MD, USA., Tang J; Division of Viral Products, Center for Biologics Evaluation and Research (CBER), Food and Drug Administration (FDA), Silver Spring, MD, USA., Grubbs G; Division of Viral Products, Center for Biologics Evaluation and Research (CBER), Food and Drug Administration (FDA), Silver Spring, MD, USA., Stein S; Emerging Pathogens Section, Critical Care Medicine Department, Clinical Center (CC), NIH, Bethesda, MD, USA.; Laboratory of Immunoregulation, NIAID, NIH, Bethesda, MD, USA., Ramelli S; Emerging Pathogens Section, Critical Care Medicine Department, Clinical Center (CC), NIH, Bethesda, MD, USA., Kabat J; Center for Advanced Tissue Imaging, LISB, NIAID, NIH, Bethesda, MD, USA., Behzadpour H; Division of Pediatric Otolaryngology, Children's National Hospital, Washington, DC, USA., Karkanitsa M; Laboratory of Immuno-Engineering, National Institute of Biomedical Imaging and Bioengineering (NIBIB), NIH, Bethesda, MD, USA., Spathies J; Trans-NIH Shared Resource on Biomedical Engineering and Physical Science, NIBIB, NIH, Bethesda, MD, USA., Kalish H; Trans-NIH Shared Resource on Biomedical Engineering and Physical Science, NIBIB, NIH, Bethesda, MD, USA., Kardava L; B-cell Immunology Section, Laboratory of Immunoregulation, NIAID, NIH, Bethesda, MD, USA., Kirby M; National Human Genome Research Institute (NHGRI), NIH, Bethesda, MD, USA., Cheung F; Center for Human Immunology, NIAID, NIH, Bethesda, MD, USA., Preite S; Cell Signaling and Immunity Section, Laboratory of Immune System Biology (LISB), National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD, USA., Duncker PC; Cytek Biosciences, Fremont, CA, USA., Kitakule MM; Laboratory of Allergic Diseases, NIAID, NIH, Bethesda, MD, USA., Romero N; Division of Otolaryngology, Department of Surgery, George Washington University School of Medicine and Health Sciences, Washington, DC, USA., Preciado D; Division of Pediatric Otolaryngology, Children's National Hospital, Washington, DC, USA.; Division of Otolaryngology, Department of Surgery, George Washington University School of Medicine and Health Sciences, Washington, DC, USA., Gitman L; Division of Pediatric Otolaryngology, Children's National Hospital, Washington, DC, USA.; Division of Otolaryngology, Department of Surgery, George Washington University School of Medicine and Health Sciences, Washington, DC, USA., Koroleva G; Center for Human Immunology, NIAID, NIH, Bethesda, MD, USA., Smith G; Laboratory of Pathology, Center for Cancer Research, National Cancer Institute (NCI), NIH, Bethesda, MD, USA., Shaffer A; Lymphoid Malignancies Branch, Center for Cancer Research, NCI, NIH, Bethesda, MD, USA., McBain IT; Cell Signaling and Immunity Section, Laboratory of Immune System Biology (LISB), National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD, USA., McGuire PJ; National Human Genome Research Institute (NHGRI), NIH, Bethesda, MD, USA., Pittaluga S; Laboratory of Pathology, Center for Cancer Research, National Cancer Institute (NCI), NIH, Bethesda, MD, USA., Germain RN; Center for Advanced Tissue Imaging, LISB, NIAID, NIH, Bethesda, MD, USA.; Lymphocyte Biology Section, LISB, NIAID, NIH, Bethesda, MD, USA., Apps R; Center for Human Immunology, NIAID, NIH, Bethesda, MD, USA., Schwartz DM; Laboratory of Allergic Diseases, NIAID, NIH, Bethesda, MD, USA., Sadtler K; Laboratory of Immuno-Engineering, National Institute of Biomedical Imaging and Bioengineering (NIBIB), NIH, Bethesda, MD, USA., Moir S; B-cell Immunology Section, Laboratory of Immunoregulation, NIAID, NIH, Bethesda, MD, USA., Chertow DS; Emerging Pathogens Section, Critical Care Medicine Department, Clinical Center (CC), NIH, Bethesda, MD, USA.; Laboratory of Immunoregulation, NIAID, NIH, Bethesda, MD, USA., Kleinstein SH; Department of Pathology, Yale School of Medicine, New Haven, CT, USA.; Program in Computational Biology and Bioinformatics, Yale University, New Haven, CT, USA.; Department of Immunobiology, Yale School of Medicine, New Haven, CT, USA., Khurana S; Division of Viral Products, Center for Biologics Evaluation and Research (CBER), Food and Drug Administration (FDA), Silver Spring, MD, USA., Tsang JS; Center for Human Immunology, NIAID, NIH, Bethesda, MD, USA.; Multiscale Systems Biology Section, LISB, NIAID, NIH, Bethesda, MD, USA., Mudd P; Division of Pediatric Otolaryngology, Children's National Hospital, Washington, DC, USA.; Division of Otolaryngology, Department of Surgery, George Washington University School of Medicine and Health Sciences, Washington, DC, USA., Schwartzberg PL; Cell Signaling and Immunity Section, Laboratory of Immune System Biology (LISB), National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD, USA. pams@nih.gov.; National Human Genome Research Institute (NHGRI), NIH, Bethesda, MD, USA. pams@nih.gov., Manthiram K; Cell Signaling and Immunity Section, Laboratory of Immune System Biology (LISB), National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD, USA. kalpana.manthiram@nih.gov.
Jazyk:	angličtina
Zdroj:	Nature immunology [Nat Immunol] 2023 Jan; Vol. 24 (1), pp. 186-199. Date of Electronic Publication: 2022 Dec 19.
DOI:	10.1038/s41590-022-01367-z
Abstrakt:	Most studies of adaptive immunity to SARS-CoV-2 infection focus on peripheral blood, which may not fully reflect immune responses at the site of infection. Using samples from 110 children undergoing tonsillectomy and adenoidectomy during the COVID-19 pandemic, we identified 24 samples with evidence of previous SARS-CoV-2 infection, including neutralizing antibodies in serum and SARS-CoV-2-specific germinal center and memory B cells in the tonsils and adenoids. Single-cell B cell receptor (BCR) sequencing indicated virus-specific BCRs were class-switched and somatically hypermutated, with overlapping clones in the two tissues. Expanded T cell clonotypes were found in tonsils, adenoids and blood post-COVID-19, some with CDR3 sequences identical to previously reported SARS-CoV-2-reactive T cell receptors (TCRs). Pharyngeal tissues from COVID-19-convalescent children showed persistent expansion of germinal center and antiviral lymphocyte populations associated with interferon (IFN)-γ-type responses, particularly in the adenoids, and viral RNA in both tissues. Our results provide evidence for persistent tissue-specific immunity to SARS-CoV-2 in the upper respiratory tract of children after infection. (© 2022. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu Full text from SpringerLink