Circulating adiponectin levels, expression of adiponectin receptors, and methylation of adiponectin gene promoter in relation to Alzheimer's disease.

Autor: Kaiyrlykyzy A; Center for Life Sciences, National Laboratory Astana, Nazarbayev University, 53 Kabanbay Batyr Avenue, Astana, Kazakhstan.; Faculty of Medicine and Public Health, al-Farabi Kazakh National University, Almaty, Kazakhstan., Umbayev B; Center for Life Sciences, National Laboratory Astana, Nazarbayev University, 53 Kabanbay Batyr Avenue, Astana, Kazakhstan., Masoud AR; Center for Life Sciences, National Laboratory Astana, Nazarbayev University, 53 Kabanbay Batyr Avenue, Astana, Kazakhstan.; Louisiana Tech University, Ruston, LA, USA., Baibulatova A; Center for Life Sciences, National Laboratory Astana, Nazarbayev University, 53 Kabanbay Batyr Avenue, Astana, Kazakhstan., Tsoy A; Center for Life Sciences, National Laboratory Astana, Nazarbayev University, 53 Kabanbay Batyr Avenue, Astana, Kazakhstan., Olzhayev F; Center for Life Sciences, National Laboratory Astana, Nazarbayev University, 53 Kabanbay Batyr Avenue, Astana, Kazakhstan., Alzhanova D; Department of Neurology and Psychiatry, Astana Medical University, Astana, Kazakhstan.; Open Clinic, Astana, Kazakhstan., Zholdasbekova G; Karaganda State Medical University, Karaganda, Kazakhstan., Davletov K; Asfendiyarov Kazakh National Medical University, Almaty, Kazakhstan., Akilzhanova A; Center for Life Sciences, National Laboratory Astana, Nazarbayev University, 53 Kabanbay Batyr Avenue, Astana, Kazakhstan., Askarova S; Center for Life Sciences, National Laboratory Astana, Nazarbayev University, 53 Kabanbay Batyr Avenue, Astana, Kazakhstan. shaskarova@nu.edu.kz.
Jazyk: angličtina
Zdroj: BMC medical genomics [BMC Med Genomics] 2022 Dec 16; Vol. 15 (1), pp. 262. Date of Electronic Publication: 2022 Dec 16.
DOI: 10.1186/s12920-022-01420-8
Abstrakt: Background: The role of adiponectin (ADIPOQ) in Alzheimer's disease (AD) has been documented, however, demonstrating controversial results. In this study, we investigated blood serum ADIPOQ levels, methylation of the adiponectin gene promoter, and adiponectin receptors (AdipoR1 and AdipoR2) expression in blood samples isolated from AD patients and healthy controls.
Methods: We performed a case-control study including 248 subjects (98 AD patients and 150 healthy controls); ADIPOQ serum levels, AdipoR1, and AdipoR2 levels in PBMC were measured by ELISA Kits, and ADIPOQ gene methylation was analyzed using methyl-specific PCR.
Results: Serum adiponectin levels were threefold higher in the AD group compared to the controls. We have also found a positive correlation between adiponectin and MMSE scores and high-density lipoprotein cholesterol (HDL-C) in AD patients. A significant difference in the proportion of methylation of the CpG sites at - 74 nt of the ADIPOQ gene promoter was detected in AD cases, and the levels of adiponectin in blood serum were significantly higher in methylated samples in the AD group compared to controls. The amount of AdipoR1 was significantly higher among AD subjects, while the expression of AdipoR2 did not vary between AD patients and controls.
Conclusion: These findings may contribute to a deeper understanding of the etiological factors leading to the development of dementia and may serve as a basis for the development of predictive biomarkers of AD.
(© 2022. The Author(s).)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje