Macrophagic AMPKα1 orchestrates regenerative inflammation induced by glucocorticoids.

Autor: Caratti G; Institute of Comparative Molecular Endocrinology, Universität Ulm, Ulm, Germany., Desgeorges T; Institut NeuroMyoGène, Université Claude Bernard Lyon 1, CNRS UMR 5310, INSERM U1217, Université de Lyon, Lyon, France., Juban G; Institut NeuroMyoGène, Université Claude Bernard Lyon 1, CNRS UMR 5310, INSERM U1217, Université de Lyon, Lyon, France., Stifel U; Institute of Comparative Molecular Endocrinology, Universität Ulm, Ulm, Germany., Fessard A; Institut NeuroMyoGène, Université Claude Bernard Lyon 1, CNRS UMR 5310, INSERM U1217, Université de Lyon, Lyon, France., Koenen M; Institute of Comparative Molecular Endocrinology, Universität Ulm, Ulm, Germany., Caratti B; Institute of Comparative Molecular Endocrinology, Universität Ulm, Ulm, Germany., Théret M; Institut NeuroMyoGène, Université Claude Bernard Lyon 1, CNRS UMR 5310, INSERM U1217, Université de Lyon, Lyon, France., Skurk C; Department of Cardiology, Charité Universitätsmedizin Berlin, Berlin, Germany.; Franklin/German Centre for Cardiovascular Research (DZHK), Partner Site Berlin/Institute of Health (BIH), Berlin, Germany., Chazaud B; Institut NeuroMyoGène, Université Claude Bernard Lyon 1, CNRS UMR 5310, INSERM U1217, Université de Lyon, Lyon, France., Tuckermann JP; Institute of Comparative Molecular Endocrinology, Universität Ulm, Ulm, Germany., Mounier R; Institut NeuroMyoGène, Université Claude Bernard Lyon 1, CNRS UMR 5310, INSERM U1217, Université de Lyon, Lyon, France.
Jazyk: angličtina
Zdroj: EMBO reports [EMBO Rep] 2023 Feb 06; Vol. 24 (2), pp. e55363. Date of Electronic Publication: 2022 Dec 15.
DOI: 10.15252/embr.202255363
Abstrakt: Macrophages are key cells after tissue damage since they mediate both acute inflammatory phase and regenerative inflammation by shifting from pro-inflammatory to restorative cells. Glucocorticoids (GCs) are the most potent anti-inflammatory hormone in clinical use, still their actions on macrophages are not fully understood. We show that the metabolic sensor AMP-activated protein kinase (AMPK) is required for GCs to induce restorative macrophages. GC Dexamethasone activates AMPK in macrophages and GC receptor (GR) phosphorylation is decreased in AMPK-deficient macrophages. Loss of AMPK in macrophages abrogates the GC-induced acquisition of their repair phenotype and impairs GC-induced resolution of inflammation in vivo during post-injury muscle regeneration and acute lung injury. Mechanistically, two categories of genes are impacted by GC treatment in macrophages. Firstly, canonical cytokine regulation by GCs is not affected by AMPK loss. Secondly, AMPK-dependent GC-induced genes required for the phenotypic transition of macrophages are co-regulated by the transcription factor FOXO3, an AMPK substrate. Thus, beyond cytokine regulation, GR requires AMPK-FOXO3 for immunomodulatory actions in macrophages, linking their metabolic status to transcriptional control in regenerative inflammation.
(© 2022 The Authors. Published under the terms of the CC BY NC ND 4.0 license.)
Databáze: MEDLINE
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