Tagging active neurons by soma-targeted Cal-Light.

Autor: Hyun JH; Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA.; Max Planck Florida Institute for Neuroscience, Jupiter, FL, 33458, USA.; Department of Brain Sciences, DGIST, Daegu, Republic of Korea., Nagahama K; Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA., Namkung H; Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA., Mignocchi N; Max Planck Florida Institute for Neuroscience, Jupiter, FL, 33458, USA., Roh SE; Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA., Hannan P; Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA.; Max Planck Florida Institute for Neuroscience, Jupiter, FL, 33458, USA., Krüssel S; Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA.; Max Planck Florida Institute for Neuroscience, Jupiter, FL, 33458, USA., Kwak C; Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA., McElroy A; Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA., Liu B; Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA., Cui M; Department of Anatomy, Korea University College of Medicine, Seoul, Republic of Korea.; BK21 Graduate Program, Department of Biomedical Sciences, Korea University College of Medicine, Seoul, Republic of Korea., Lee S; Department of Anatomy, Korea University College of Medicine, Seoul, Republic of Korea.; BK21 Graduate Program, Department of Biomedical Sciences, Korea University College of Medicine, Seoul, Republic of Korea., Lee D; Department of Anatomy, Korea University College of Medicine, Seoul, Republic of Korea.; BK21 Graduate Program, Department of Biomedical Sciences, Korea University College of Medicine, Seoul, Republic of Korea., Huganir RL; Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA., Worley PF; Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA., Sawa A; Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA.; Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA.; Department of Psychiatry, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA.; Department of Genetic Medicine, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA.; Department of Pharmacology, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA.; Department of Mental Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, 21025, USA., Kwon HB; Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA. hkwon29@jhmi.edu.; Max Planck Florida Institute for Neuroscience, Jupiter, FL, 33458, USA. hkwon29@jhmi.edu.; Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA. hkwon29@jhmi.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2022 Dec 12; Vol. 13 (1), pp. 7692. Date of Electronic Publication: 2022 Dec 12.
DOI: 10.1038/s41467-022-35406-y
Abstrakt: Verifying causal effects of neural circuits is essential for proving a direct circuit-behavior relationship. However, techniques for tagging only active neurons with high spatiotemporal precision remain at the beginning stages. Here we develop the soma-targeted Cal-Light (ST-Cal-Light) which selectively converts somatic calcium rise triggered by action potentials into gene expression. Such modification simultaneously increases the signal-to-noise ratio of reporter gene expression and reduces the light requirement for successful labeling. Because of the enhanced efficacy, the ST-Cal-Light enables the tagging of functionally engaged neurons in various forms of behaviors, including context-dependent fear conditioning, lever-pressing choice behavior, and social interaction behaviors. We also target kainic acid-sensitive neuronal populations in the hippocampus which subsequently suppress seizure symptoms, suggesting ST-Cal-Light's applicability in controlling disease-related neurons. Furthermore, the generation of a conditional ST-Cal-Light knock-in mouse provides an opportunity to tag active neurons in a region- or cell-type specific manner via crossing with other Cre-driver lines. Thus, the versatile ST-Cal-Light system links somatic action potentials to behaviors with high temporal precision, and ultimately allows functional circuit dissection at a single cell resolution.
(© 2022. The Author(s).)
Databáze: MEDLINE
Externí odkaz: