Morphological investigations of endomorphin-2 and spinoparabrachial projection neurons in the spinal dorsal horn of the rat.

Autor:	Yin JB; Department of Human Anatomy, K. K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an, China.; Department of Neurology, The 960th Hospital of Joint Logistics Support, PLA, Jinan, China.; State Key Laboratory of Military Medical Psychology, The Fourth Military Medical University, Xi'an, China., Lu YC; Department of Human Anatomy, K. K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an, China., Li F; Department of Human Anatomy, K. K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an, China., Zhang T; Department of Human Anatomy, K. K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an, China., Ding T; Institute of Orthopedics, Xijing Hospital, The Fourth Military Medical University, Xi'an, China., Hu HQ; Department of Neurology, The 960th Hospital of Joint Logistics Support, PLA, Jinan, China.; State Key Laboratory of Military Medical Psychology, The Fourth Military Medical University, Xi'an, China., Chen YB; Department of Human Anatomy, Fujian Health College, Fuzhou, China., Guo HW; Department of Neurology, The 960th Hospital of Joint Logistics Support, PLA, Jinan, China., Kou ZZ; Department of Human Anatomy, K. K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an, China., Zhang MM; Department of Human Anatomy, K. K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an, China., Yuan J; Department of Neurology, The 960th Hospital of Joint Logistics Support, PLA, Jinan, China., Chen T; Department of Human Anatomy, K. K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an, China., Li H; Department of Human Anatomy, K. K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an, China., Cao BZ; Department of Neurology, The 960th Hospital of Joint Logistics Support, PLA, Jinan, China., Dong YL; Department of Human Anatomy, K. K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an, China., Li YQ; Department of Human Anatomy, K. K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an, China.
Jazyk:	angličtina
Zdroj:	Frontiers in neuroanatomy [Front Neuroanat] 2022 Nov 23; Vol. 16, pp. 1072704. Date of Electronic Publication: 2022 Nov 23 (Print Publication: 2022).
DOI:	10.3389/fnana.2022.1072704
Abstrakt:	It has been proved that endomorphin-2 (EM2) produced obvious analgesic effects in the spinal dorsal horn (SDH), which existed in our human bodies with remarkable affinity and selectivity for the μ-opioid receptor (MOR). Our previous study has demonstrated that EM2 made synapses with the spinoparabrachial projection neurons (PNs) in the SDH and inhibited their activities by reducing presynaptic glutamate release. However, the morphological features of EM2 and the spinoparabrachial PNs in the SDH have not been completely investigated. Here, we examined the morphological features of EM2 and the spinoparabrachial PNs by using triple fluorescence and electron microscopic immunohistochemistry. EM2-immunoreactive (-ir) afferents directly contacted with the spinoparabrachial PNs in lamina I of the SDH. Immunoelectron microscopy (IEM) were used to confirm that these contacts were synaptic connections. It was also observed that EM2-ir axon terminals contacting with spinoparabrachial PNs in lamina I contained MOR, substance P (SP) and vesicular glutamate transporter 2 (VGLUT2). In lamina II, MOR-ir neurons were observed to receive direct contacts from EM2-ir varicosities. The synaptic connections among EM2, MOR, SP, VGLUT2, and the spinoparabrachial PNs were also confirmed by IEM. In sum, our results supply morphological evidences for the analgesic effects of EM2 on the spinoparabrachial PNs in the SDH. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Yin, Lu, Li, Zhang, Ding, Hu, Chen, Guo, Kou, Zhang, Yuan, Chen, Li, Cao, Dong and Li.)
Databáze:	MEDLINE
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