Structure of the AlgKX modification and secretion complex required for alginate production and biofilm attachment in Pseudomonas aeruginosa.

Autor: Gheorghita AA; Program in Molecular Medicine, The Hospital for Sick Children, Toronto, ON, Canada.; Department of Biochemistry, University of Toronto, Toronto, ON, Canada., Li YE; Program in Molecular Medicine, The Hospital for Sick Children, Toronto, ON, Canada.; Department of Biochemistry, University of Toronto, Toronto, ON, Canada.; Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, USA., Kitova EN; Department of Chemistry, University of Alberta, Edmonton, AB, Canada., Bui DT; Department of Chemistry, University of Alberta, Edmonton, AB, Canada., Pfoh R; Program in Molecular Medicine, The Hospital for Sick Children, Toronto, ON, Canada., Low KE; Program in Molecular Medicine, The Hospital for Sick Children, Toronto, ON, Canada.; Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada., Whitfield GB; Program in Molecular Medicine, The Hospital for Sick Children, Toronto, ON, Canada.; Department of Biochemistry, University of Toronto, Toronto, ON, Canada.; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montréal, QC, Canada., Walvoort MTC; Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.; Department of Chemical Biology, Stratingh Institute for Chemistry, University of Groningen, Groningen, The Netherlands., Zhang Q; Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.; National Research Centre for Carbohydrate Synthesis, Jiangxi Normal University, Nanchang, China., Codée JDC; Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands., Klassen JS; Department of Chemistry, University of Alberta, Edmonton, AB, Canada., Howell PL; Program in Molecular Medicine, The Hospital for Sick Children, Toronto, ON, Canada. howell@sickkids.ca.; Department of Biochemistry, University of Toronto, Toronto, ON, Canada. howell@sickkids.ca.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2022 Dec 09; Vol. 13 (1), pp. 7631. Date of Electronic Publication: 2022 Dec 09.
DOI: 10.1038/s41467-022-35131-6
Abstrakt: Synthase-dependent secretion systems are a conserved mechanism for producing exopolysaccharides in Gram-negative bacteria. Although widely studied, it is not well understood how these systems are organized to coordinate polymer biosynthesis, modification, and export across both membranes and the peptidoglycan. To investigate how synthase-dependent secretion systems produce polymer at a molecular level, we determined the crystal structure of the AlgK-AlgX (AlgKX) complex involved in Pseudomonas aeruginosa alginate exopolysaccharide acetylation and export. We demonstrate that AlgKX directly binds alginate oligosaccharides and that formation of the complex is vital for polymer production and biofilm attachment. Finally, we propose a structural model for the AlgEKX outer membrane modification and secretion complex. Together, our study provides insight into how alginate biosynthesis proteins coordinate production of a key exopolysaccharide involved in establishing persistent Pseudomonas lung infections.
(© 2022. The Author(s).)
Databáze: MEDLINE
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