Integration of in vitro and in-silico analysis of Caulerpa racemosa against antioxidant, antidiabetic, and anticancer activities.

Autor: Dissanayake IH; Department of Zoology, University of Sri Jayewardenepura, Nugegoda, 10250, Sri Lanka., Bandaranayake U; Department of Zoology, University of Sri Jayewardenepura, Nugegoda, 10250, Sri Lanka., Keerthirathna LR; Department of Zoology, University of Sri Jayewardenepura, Nugegoda, 10250, Sri Lanka., Manawadu C; Department of Zoology, University of Sri Jayewardenepura, Nugegoda, 10250, Sri Lanka., Silva RM; Department of Statistics, University of Sri Jayewardenepura, Nugegoda, 10250, Sri Lanka., Mohamed B; Light and Electron Microscopy Unit, Medical Core Facility and Research Platforms, King Abdullah International Medical Research Centre, National Guard Health Affairs, Mail Code 1515, P.O. Box 3660, Riyadh, 11481, Saudi Arabia., Ali R; King Abdullah International Medical Research Center (KAIMRC), Medical Research Core Facility and Platforms (MRCFP), King Saud bin Abdulaziz University for Health Sciences (KSAU-HS), Ministry of National Guard Health Affairs (MNGHA), Riyadh, 11481, Saudi Arabia., Peiris DC; Department of Zoology/Genetics and Molecular Biology Unit (Centre for Biotechnology, University of Sri Jayewardenepura, Nugegoda, 10250, Sri Lanka. dinithi@sci.sjp.ac.lk.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Dec 02; Vol. 12 (1), pp. 20848. Date of Electronic Publication: 2022 Dec 02.
DOI: 10.1038/s41598-022-24021-y
Abstrakt: Marine algae are found to be excellent in their nutritional and potential therapeutic properties. This study explores the antidiabetic and anticancer potential of fractionated polyphenolic extract of Caulerpa racemosa, green macroalgae. Crude polyphenolic extract (CPE) of C. racemosa and its fractions (n-hexane, ethyl acetate, chloroform, and distilled water) were tested for its total phenol and flavonoid contents and antioxidant potential. The ethyl acetate fraction was subjected to gas chromatography/mass spectrometry (GC/MS). The in vitro antidiabetic activity was assessed by alpha-amylase, glucosidase inhibition and anti-glycation assays. Also, in-silico studies were conducted to test the binding affinities between caulerpin with alpha-glucosidase enzyme and estrogen receptor (ER) active sites. Each fraction was tested for its in vitroin vitroanticancer activity by CellTiter-Glo and MTT cell proliferation assays. The total phenolic and flavonoid contents and the antioxidant potential of the crude extract were observed to be dose dependent. The GC/MS analysis of the ethyl acetate fraction yielded 47 peaks, whereas n-hexadecanoic acid and hexadecanoic acid methyl ester showed the highest compatibility percentages of 99% and 96%, respectively. The CPE exhibited a higher potential in both alpha-amylase inhibitory and anti-glycation activities. The ethyl acetate fraction was more effective against alpha-glucosidase inhibition. Molecular docking revealed a high binding affinity between the alpha-glucosidase enzyme and caulerpin and showed high binding affinity toward caulerpin, with H-bond interactions. The in vitro anticancer analyses revealed that chloroform fraction and CPE exhibited moderate activity on the KAIMRC1 cell line. Also, the CPE exhibited high specificity compared to the standard drug in anticancer studies. Our findings evidence the pharmacological potential of the CPE of C. racemosa, and bioactive compounds of the species may be utilized as lead molecules to develop anti-diabetic and anti-cancer drugs.
(© 2022. The Author(s).)
Databáze: MEDLINE
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