Reproductive genetics laboratory may impact euploid blastocyst and live birth rates: a comparison of 4 national laboratories' PGT-A results from vitrified donor oocytes.

Autor:	Bardos J; National Institutes of Health, Bethesda, Maryland; Walter Reed National Military Medical Center, Bethesda, Maryland. Electronic address: bardosmd@gmail.com., Kwal J; Department of Obstetrics and Gynecology, University of Miami Miller School of Medicine, Miami, Florida., Caswell W; Donor Egg Bank USA, Rockville, Maryland., Jahandideh S; Shady Grove Fertility, Rockville, Maryland., Stratton M; Donor Egg Bank USA, Rockville, Maryland., Tucker M; Shady Grove Fertility, Rockville, Maryland., DeCherney A; National Institutes of Health, Bethesda, Maryland., Devine K; Shady Grove Fertility, Rockville, Maryland., Hill M; Walter Reed National Military Medical Center, Bethesda, Maryland., O'Brien JE; Donor Egg Bank USA, Rockville, Maryland.
Jazyk:	angličtina
Zdroj:	Fertility and sterility [Fertil Steril] 2023 Jan; Vol. 119 (1), pp. 29-35. Date of Electronic Publication: 2022 Nov 29.
DOI:	10.1016/j.fertnstert.2022.10.010
Abstrakt:	Objective: To evaluate potential variation in the euploid blastocyst rate and live birth rate (LBR) per single frozen euploid blastocyst transfer, among 4 unique United States reproductive genetics laboratories. Analyses were limited to blastocysts derived from vitrified donor oocytes, to minimize variation in oocyte quality. Design: Retrospective cohort study from 2016 to 2020. Setting: Donor Egg Bank Database. Patient(s): Patients undergoing in vitro fertilization with donor oocytes. We excluded patients with uterine factor, male factor, or surgically extracted sperm. Only healthy women <34 years old were accepted as oocyte donors. Intervention(s): Next generation sequencing platforms for chromosomal analysis MAIN OUTCOME MEASURE(S): Euploid blastocyst rate and LBR per euploid transfer. Secondary outcomes included the rate of aneuploidy, mosaic calls, biochemical pregnancy loss, and miscarriage rate. Result(s): A total of 2,633 embryos were included. Four laboratories had >200 embryos tested. Euploid blastocyst rate was significantly higher in laboratory A (73.6%) vs. B (63.3%), C (60.9%), and D (52.3%). Mosaic rate was significantly lower for Laboratories B (2.8%) and C (5.5%) vs. Laboratories A (9.9%) and D (11.5). The LBR was significantly higher in laboratory A (58.73%) vs. laboratory D (47.3%). There were no significant differences in the implantation or miscarriage rate among laboratories. Conclusion(s): In this large study, controlling for oocyte quality, some preimplantation genetic testing for aneuploidy (PGT-A) laboratories report a significantly higher euploid blastocyst rate with concurrent higher LBR. This type of comparison is important as it provides insight into the role of the PGT-A process in outcomes. Further research is needed to evaluate the differences in laboratory techniques and bioinformatic algorithms accounting for variable outcomes across PGT-A laboratories. (Copyright © 2022 American Society for Reproductive Medicine. All rights reserved.)
Databáze:	MEDLINE
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