Molecular characterization of BK virus detected in renal transplant patients in Sri Lanka: a preliminary study.

Autor: Yashodha Ratnayake AKDV; Institute of Biochemistry, Molecular Biology & Biotechnology, University of Colombo, Colombo, Sri Lanka., Fernando N; Institute of Biochemistry, Molecular Biology & Biotechnology, University of Colombo, Colombo, Sri Lanka., Gajanayake T; Faculty of Science, Horizon Campus, Colombo, Sri Lanka., Handunnetti SM; Institute of Biochemistry, Molecular Biology & Biotechnology, University of Colombo, Colombo, Sri Lanka., Jude Jayamaha SC; Department of Virology, National Influenza Centre, Medical Research Institute, Colombo, Sri Lanka.
Jazyk: angličtina
Zdroj: The Indian journal of medical research [Indian J Med Res] 2022 Sep; Vol. 156 (3), pp. 500-507.
DOI: 10.4103/ijmr.IJMR_79_20
Abstrakt: Background & Objectives: BK virus (BKV) is a polyomavirus and cause of a common infection after renal transplantation which could be preceded to BKV-associated nephropathy. It has four main subtypes (I-IV). BKV subtypes II and III are rare, whereas subtype I shows a ubiquitous distribution. The objective of the present study was to investigate the prevailing BKV subtypes and subgroups in renal transplant patients in Sri Lanka.
Methods: The presence of BKV in urine was tested through virus load quantification by real-time PCR from 227 renal transplant patients who were suspected to have BKV infection. Of these patients only 41 were found to be BKV infected (>10 3 copies/ml) and those were subjected to conventional PCR amplification of VP1 gene followed by BKV genotyping via phylogenetic analysis based on DNA sequencing data.
Results: Persistent BK viral loads varied from 1×10 3 to 3×10 8 copies/ml. Of the 41 patient samples, 25 gave positive results for PCR amplification of subtyping region of VP1 gene of BKV. BKV genotyping resulted in detecting subtype I in 18 (72%) and subtype II in seven (28%) patients. BKV subgroups of Ia, Ib-1 and Ib-11, and Ic were identified with frequencies of 6/18 (33.3%), 6/18 (33.3%), 5/18 (27.8%), and 1/18 (5.6%), respectively.
Interpretation & Conclusions: Findings from this preliminary study showed a high occurrence of subtype I, while the presence of subtype II, which is rare and less prevalent, was a novel finding for this Asian region. This emphasizes the need for further molecular and serological studies to determine the prevalence of different BKV subtypes in Sri Lanka.
Databáze: MEDLINE
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