County-wide assessments of Illinois white-tailed deer (Odocoileus virginianus) prion protein gene variation using improved primers and potential implications for management.

Autor: Raudabaugh DB; Illinois Natural History Survey, University of Illinois, Champaign, Illinois, United States of America., Ishida Y; Department of Animal Science, University of Illinois, Urbana, Illinois, United States of America., Haley NJ; Department of Microbiology and Immunology, Midwestern University, Glendale, AZ, United States of America., Brown WM; Department of Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana, Illinois, United States of America., Novakofski J; Illinois Natural History Survey, University of Illinois, Champaign, Illinois, United States of America.; Department of Animal Science, University of Illinois, Urbana, Illinois, United States of America., Roca AL; Illinois Natural History Survey, University of Illinois, Champaign, Illinois, United States of America.; Department of Animal Science, University of Illinois, Urbana, Illinois, United States of America.; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America., Mateus-Pinilla NE; Illinois Natural History Survey, University of Illinois, Champaign, Illinois, United States of America.; Department of Animal Science, University of Illinois, Urbana, Illinois, United States of America.; Department of Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana, Illinois, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2022 Nov 30; Vol. 17 (11), pp. e0274640. Date of Electronic Publication: 2022 Nov 30 (Print Publication: 2022).
DOI: 10.1371/journal.pone.0274640
Abstrakt: Chronic wasting disease (CWD) is a fatal, highly infectious prion disease that affects captive and wild cervids. Chronic wasting disease is the only known transmissible spongiform encephalopathy affecting free-ranging wildlife. In CWD-positive deer, some haplotypes of the prion protein gene PRNP are detected at lower frequencies as compared to CWD-negative deer, as are some variants of the prion protein PrP. Here, we examined wild, hunter-harvested CWD-negative white-tailed deer (Odocoileus virginianus) to determine whether there were geographical or temporal differences in the PRNP haplotypes, PRNP diplotypes, PrP proteoforms, and in the proportion of deer with at least one protective haplotype. We sampled 96-100 hunter-harvested deer per county at two time points in the Illinois counties of Jo Daviess, LaSalle, and Winnebago, chosen based on their geographic locations and known occurrence of CWD. The entire coding region of PRNP was sequenced, with haplotypes, diplotypes, and PrP proteoforms inferred. Across time, in Winnebago there was a significant increase in PrP proteoform F (p = 0.034), which is associated with a lower vulnerability to CWD. In every county, there was an increase over time in the frequency of deer carrying at least one protective haplotype to CWD, with a significant increase (p = 0.02) in the Jo Daviess County CWD infected region. We also found that primer combination was important as there was an 18.7% difference in the number of the deer identified as homozygous depending on primer usage. Current Illinois state management practices continue to remove CWD infected deer from locally infected areas helping to keep CWD prevalence low. Nonetheless, continued research on spatial and temporal changes in PRNP haplotypes, PrP proteoforms, and levels of deer vulnerability among Illinois deer will be important for the management of CWD within the state of Illinois and beyond.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2022 Raudabaugh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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