Phyto-Extract-Mediated Synthesis of Silver Nanoparticles (AgNPs) and Their Biological Activities.

Autor: Adnan M; Department of Botany, Kohat University of Science and Technology, Kohat 26000, Pakistan., Akbar A; Department of Botany, Kohat University of Science and Technology, Kohat 26000, Pakistan., Mussarat S; Department of Botany, Kohat University of Science and Technology, Kohat 26000, Pakistan., Murad W; Department of Botany, Abdul Wali Khan University Mardan, Pakistan., Hameed I; Department of Botany, University of Chitral, Chitral 17200, Pakistan., Begum S; Department of Environmental Sciences, Fatima Jinnah Women University, Rawalpindi, Pakistan., Nazir R; Department of Chemistry, Kohat University of Science and Technology, Kohat 26000, Pakistan., Ali N; Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat 26000, Pakistan., Ali EA; Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia., Bari A; Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia., Aziz MA; Department of Environmental Sciences, Information and Statistics, Ca Foscari University of Venice, Vio Tornio 155, 30172 Venezia, Italy., Khan SN; Department of Zoology, Kohat University of Science and Technology, Kohat 26000, Pakistan.
Jazyk: angličtina
Zdroj: BioMed research international [Biomed Res Int] 2022 Nov 16; Vol. 2022, pp. 9845022. Date of Electronic Publication: 2022 Nov 16 (Print Publication: 2022).
DOI: 10.1155/2022/9845022
Abstrakt: Background: Nanotechnology finds broad applications in the field of nanomedicine, an emerging new field used for diagnosis, treatment, prevention of diseases, and improvement of health.
Objectives: To synthesize silver nanoparticles (AgNPs) from Withania somnifera and Fagonia indica and to carry out their antimicrobial, insecticidal, and phytotoxic activities, a step toward the new range of nanomedicines.
Methods: Silver nanoparticles were synthesized from Withania somnifera and Fagonia indica by chemical reduction method, and further biological activities of these nanoparticles were compared with crude methanolic extract, prepared through cold maceration process, at the concentration of 50 mg/ml.
Results: Among all tested bacterial pathogens, crude extract of W. somnifera showed a statistically high significant inhibition zone in millimeter against Pseudomonas aeruginosa (21; p < 0.01). AgNPs showed highly significant result against Streptococcus pneumonia (14; p < 0.01). In comparison with crude extracts, AgNPs showed statistically significant ( p < 0.01) results against S. pneumonia (AgNPs, 14; crude, 8.33 mm). Crude extract showed significant inhibition zone against two bacterial strains, P. aeruginosa (crude, 21; AgNPs, 11.67 mm) and Klebsiella pneumoniae (crude, 11.33; AgNPs, 8 mm). Crude extracts of F. indica showed the significant activity against Vibrio cholera ( p < 0.01; 11.33 mm). Silver nanoparticles of F. indica exhibited the highest significant activity against Aspergillus flavus and Fusarium oxysporum while AgNPs of W. somnifera were active only against A. flavus . Extracts of W. somnifera and F. indica showed increasing phytotoxic activity with increasing concentrations. The highest significant inhibition was obtained for crude extract (46.7) and AgNPs (45.7) of F. indica at 1000  μ g/ml. Insecticidal activity of crude and AgNPs of both plants showed significant inhibition against all tested insects with increasing time intervals, and the highest significant result was obtained at 72 hours with a value of p < 0.01 except T. castaneum .
Conclusions: Both crude and AgNPs showed potent activity; however, in comparison, silver nanoparticles showed slightly enhanced activity. Crude and AgNPs of both plants showed good phytotoxic and insecticidal inhibition. Antimicrobial studies of AgNPs on diseases causing pathogens open a door for new antimicrobial agents and could be the answer to antibiotic resistance after further analysis.
Competing Interests: The authors declare that they have no conflicts of interest.
(Copyright © 2022 Muhammad Adnan et al.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje