Bioactivity Potential of Bioceramic-Based Root Canal Sealers: A Scoping Review.

Autor:	Estivalet MS; Program in Dentistry, School of Dentistry, Federal University of Pelotas (UFPEL), Pelotas 96010-610, RS, Brazil., de Araújo LP; Department of Restorative Dentistry, Division of Endodontics, Piracicaba Dental School, State University of Campinas (UNICAMP), Piracicaba 13083-970, SP, Brazil., Immich F; Program in Dentistry, School of Dentistry, Federal University of Pelotas (UFPEL), Pelotas 96010-610, RS, Brazil., da Silva AF; Program in Dentistry, School of Dentistry, Federal University of Pelotas (UFPEL), Pelotas 96010-610, RS, Brazil.; Department of Restorative Dentistry, School of Dentistry, Federal University of Pelotas (UFPEL), Pelotas 96015-560, RS, Brazil., Ferreira NS; Program in Dentistry, School of Dentistry, Federal University of Pelotas (UFPEL), Pelotas 96010-610, RS, Brazil.; Department of Semiology and Clinics, School of Dentistry, Federal University of Pelotas (UFPEL), Pelotas 96015-560, RS, Brazil., da Rosa WLO; Program in Dentistry, School of Dentistry, Federal University of Pelotas (UFPEL), Pelotas 96010-610, RS, Brazil.; Department of Restorative Dentistry, School of Dentistry, Federal University of Pelotas (UFPEL), Pelotas 96015-560, RS, Brazil., Piva E; Program in Dentistry, School of Dentistry, Federal University of Pelotas (UFPEL), Pelotas 96010-610, RS, Brazil.; Department of Restorative Dentistry, School of Dentistry, Federal University of Pelotas (UFPEL), Pelotas 96015-560, RS, Brazil.
Jazyk:	angličtina
Zdroj:	Life (Basel, Switzerland) [Life (Basel)] 2022 Nov 11; Vol. 12 (11). Date of Electronic Publication: 2022 Nov 11.
DOI:	10.3390/life12111853
Abstrakt:	Introduction: Bioceramic-based root canal sealers are novel materials with a bioactivity potential that stands out compared with conventional root canal sealers. However, the term bioactivity may be overused and is often misunderstood. Hence, the objective of this study was to synthesize and map key concepts related to the bioactivity analysis of bioceramic-based root canal sealers. Methods: The present scoping review is reported in accordance with the PRISMA-ScR Statement and is registered in the Open Science Framework. Two blinded reviewers carried out a comprehensive search in six databases up to January 10th, 2022: MEDLINE, Scopus, Embase, Web of Science, Cochrane Library, and Lilacs/BBO. Eligibility was considered for in vitro and in vivo studies that evaluated the bioactivity potential of bioceramic-based root canal sealers. Results: A total of 53 studies were included in the qualitative synthesis. In vitro bioactivity was evaluated through the mineralization potential, formation of carbonated apatite on the surface, and the gene expression related to proteins involved in the mineralization process. Meanwhile, for in vivo studies, staining techniques associated with immunohistochemical tests were mainly used to detect mineralization on the material-host tissue interface. Conclusions: According to the methodology used, the most prevalent methods to assess bioactivity in acellular form were the immersion of the material in Hank's balanced salt solution, followed by surface observation with scanning electron microscopy and energy dispersive X-ray. In cell cultures, the chosen method was usually Alizarin Red staining, followed by the evaluation of alkaline phosphatase enzymatic activity and the use of molecular biology tests.
Databáze:	MEDLINE
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