No molecular evidence for influenza A virus and coronavirus in bats belonging to the families Phyllostomidae, Vespertilionidae, and Molossidae in the state of São Paulo, Brazil.

Autor: Violet-Lozano L; Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande Do Sul (UFRGS), Porto Alegre, Rio Grande Do Sul, Brazil. linaviolet14@gmail.com., Haach V; Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande Do Sul (UFRGS), Porto Alegre, Rio Grande Do Sul, Brazil., Barboza CM; Federal University of ABC, Santo André, São Paulo, Brazil.; Pasteur Institute, São Paulo, São Paulo, Brazil., Dos Santos J; Pasteur Institute, São Paulo, São Paulo, Brazil., Gomes BF; Pasteur Institute, São Paulo, São Paulo, Brazil., de Cassia Pardo de Souza T; Pasteur Institute, São Paulo, São Paulo, Brazil., Junior PC; Pasteur Institute, São Paulo, São Paulo, Brazil., Arns CW; Animal Viruses Laboratory, Department of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas (Unicamp), Campinas, São Paulo, Brazil., de Carvalho Ruthner Batista HB; Federal University of ABC, Santo André, São Paulo, Brazil.; Pasteur Institute, São Paulo, São Paulo, Brazil., Roehe PM; Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande Do Sul (UFRGS), Porto Alegre, Rio Grande Do Sul, Brazil., Franco AC; Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande Do Sul (UFRGS), Porto Alegre, Rio Grande Do Sul, Brazil.
Jazyk: angličtina
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2023 Mar; Vol. 54 (1), pp. 523-529. Date of Electronic Publication: 2022 Nov 24.
DOI: 10.1007/s42770-022-00878-z
Abstrakt: This study aimed to evaluate, by molecular methods, the presence of influenza A virus (IAV) and coronavirus in non-hematophagous bats collected in the state of São Paulo, Brazil. Samples of lung tissue and small intestine from 105 bats belonging to three families (Phyllostomidae, Vespertilionidae, and Molossidae) were collected in 22 municipalities in the state of São Paulo. Genetic identification of bats species was performed by amplification and sequencing of a fragment of 710 bp of the mitochondrial COI gene. In the detection of IAV, genomes were performed by RT-PCR, aiming at the amplification of a 245-bp fragment of the IAV matrix (M) protein gene. For coronaviruses, two fragments of 602 and 440 bp corresponding to segments along the gene encoding the RNA-dependent RNA polymerase (RdRp) were targeted. The detection limit for each of the PCRs was also determined. All samples analyzed here were negative for both viruses, and the lower limit of detection of the PCRs for the amplification of influenza virus A and coronavirus was estimated at 3.5 × 10 3 and 4.59 genomic copies per microliter, respectively. Although bats have been shown to harbor a large number of pathogens, the results of the present study support the theory that virus circulation in bats in the wild often occurs at low viral loads and that our understanding of the complex infectious dynamics of these viruses in wild conditions is still limited.
(© 2022. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
Databáze: MEDLINE
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