| Autor: |
Apostolova E; Department of Pharmacology, Toxicology, and Pharmacotherapy, Faculty of Pharmacy, Medical University-Plovdiv, Vasil Aprilov Str. 15A, 4002 Plovdiv, Bulgaria., Lukova P; Department of Pharmacognosy and Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University-Plovdiv, Vasil Aprilov Str. 15A, 4002 Plovdiv, Bulgaria., Baldzhieva A; Department of Microbiology and Immunology, Faculty of Pharmacy, Medical University-Plovdiv, Vasil Aprilov Str. 15A, 4002 Plovdiv, Bulgaria.; Research Institute at Medical University-Plovdiv, Vasil Aprilov Str. 15A, 4002 Plovdiv, Bulgaria., Delattre C; Clermont Auvergne INP, CNRS, Institut Pascal, Université Clermont Auvergne, 63000 Clermont-Ferrand, France.; Institut Universitaire de France (IUF), 1 rue Descartes, 75005 Paris, France., Molinié R; UMRT INRAE 1158 BioEcoAgro, BIOlogie des Plantes et Innovation (BIOPI), Avenue des Facultés, IUT d'Amiens, Université de Picardie Jules Verne, Le Bailly, 80025 Amiens, France., Petit E; UMRT INRAE 1158 BioEcoAgro, BIOlogie des Plantes et Innovation (BIOPI), Avenue des Facultés, IUT d'Amiens, Université de Picardie Jules Verne, Le Bailly, 80025 Amiens, France., Elboutachfaiti R; UMRT INRAE 1158 BioEcoAgro, BIOlogie des Plantes et Innovation (BIOPI), Avenue des Facultés, IUT d'Amiens, Université de Picardie Jules Verne, Le Bailly, 80025 Amiens, France., Nikolova M; Department of Biochemistry and Microbiology, Faculty of Biology, Plovdiv University Paisii Hilendarski, Tsar Asen Str. 24, 4000 Plovdiv, Bulgaria., Iliev I; Department of Biochemistry and Microbiology, Faculty of Biology, Plovdiv University Paisii Hilendarski, Tsar Asen Str. 24, 4000 Plovdiv, Bulgaria., Murdjeva M; Department of Microbiology and Immunology, Faculty of Pharmacy, Medical University-Plovdiv, Vasil Aprilov Str. 15A, 4002 Plovdiv, Bulgaria.; Research Institute at Medical University-Plovdiv, Vasil Aprilov Str. 15A, 4002 Plovdiv, Bulgaria., Kokova V; Department of Pharmacology, Toxicology, and Pharmacotherapy, Faculty of Pharmacy, Medical University-Plovdiv, Vasil Aprilov Str. 15A, 4002 Plovdiv, Bulgaria. |
| Abstrakt: |
The aim of this study was to evaluate the effects of fucoidan isolated from C. crinita on histamine-induced paw inflammation in rats, and on the serum levels of TNF-α, IL-1β, IL-6, and IL-10 in rats during systemic inflammation response. The levels of TNF-α in a model of acute peritonitis in rats were also investigated. The isolated crude fucoidan was identified as a sulfated xylogalactofucan with high, medium, and low molecular weight fractions and a content of fucose of 39.74%, xylose of 20.75%, and galactose of 15.51%. Fucoidan from C. crinita showed better anti-inflammatory effects in the rat paw edema model, and this effect was present during all stages of the experiment. When compared to controls, a commercial fucoidan from F. vesiculosus, the results also displayed anti-inflammatory activity on the 60th, 90th, and 120th minute of the experiment. A significant decrease in serum levels of IL-1β in rats treated with both doses of C. crinita fucoidan was observed in comparison to controls, whereas TNF-α concentrations were reduced only in the group treated with fucoidan from C. crinita at the dose of 25 mg/kg bw. In the model of carrageenan-induced peritonitis, we observed a tendency of decrease in the levels of the pro-inflammatory cytokine TNF-α in peritoneal fluid after a single dose of C. crinita fucoidan, but this did not reach the statistical significance margin. Single doses of C. crinita fucoidan did not alter serum levels of the anti-inflammatory cytokine IL-10 in animals with lipopolysaccharide-induced systemic inflammation. |
