Serum metabolomic characterization in pigs in relation to birth weight category and neonatal nutrition.

Autor:	Wellington MO; Prairie Swine Centre, Inc., Box 21057, 2105-8th Street East, Saskatoon, SK, CanadaS7H 5N9.; Department of Animal and Poultry Science, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, CanadaS7N 5A8., Rodrigues LA; Prairie Swine Centre, Inc., Box 21057, 2105-8th Street East, Saskatoon, SK, CanadaS7H 5N9.; Department of Animal and Poultry Science, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, CanadaS7N 5A8., Quinn MA; Department of Kinesiology, Michigan State University, 308 West Circle Drive, East Lansing, MI 48824, USA., Panisson JC; Department of Animal and Poultry Science, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, CanadaS7N 5A8., Ferguson DP; Department of Kinesiology, Michigan State University, 308 West Circle Drive, East Lansing, MI 48824, USA., Columbus DA; Prairie Swine Centre, Inc., Box 21057, 2105-8th Street East, Saskatoon, SK, CanadaS7H 5N9.; Department of Animal and Poultry Science, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, CanadaS7N 5A8.
Jazyk:	angličtina
Zdroj:	Journal of animal science [J Anim Sci] 2023 Jan 03; Vol. 101.
DOI:	10.1093/jas/skac386
Abstrakt:	The objective of this study was to characterize developmental differences in low birth weight (LBW) and normal birth weight (NBW) piglets with or without pre-weaning nutrient restriction using serum metabolomic profile analysis. At farrowing, 112 piglets were identified as LBW (1.22 ± 0.28 kg) or NBW (1.70 ± 0.27 kg) and were randomly assigned to receive normal nutrition (NN) or restricted nutrition (RN) (6 h/day no suckling) from days 2 to 28 post farrow (n = 8 pigs/group). On day 28, piglets were weaned onto a common diet. Fasted blood samples were obtained on days 28 and 56 (n = 8 pigs/group) and were analyzed using quantitative metabolomics via a combination of direct injection mass spectrometry with a reverse-phase LC-MS/MS custom assay. Data were normalized using logarithmic transformation and auto-scaling. Partial least squares discriminant analysis (PLS-DA) was carried out to further explore the differential metabolites among the groups (metaboanalyst.ca) with an integrated enrichment and pathway topography analysis. On day 28, LBW piglets had lower levels of essential amino acids as well as reduced metabolites associated with fatty acid oxidation, glycolysis, and the tri-carboxylic acid (TCA) cycle compared to the NBW group. The overall reduction of metabolites associated with energy production and regulation suggests that LBW vs. NBW are in an energy-survival state. On day 56, LBW pigs had increased utilization of fatty acids and resultant ketone production, evident by increased carnitines, acetoacetate, β-hydroxybutyrate, and glycerol compared to NBW pigs. In addition, compared to the NBW pigs LBW pigs had a consistent decrease in serum glucose and lactate as well as reduced TCA cycle metabolites: pyruvate, succinate, citrate, and α-ketoglutaric acid similar to day 28. Low reliance on glycolysis and the TCA cycle and higher glycerol production in the LBW pigs may indicate impairments in glucose tolerance at 56 d. In summary, LBW piglets appear to have more metabolic alterations in early life, which is not resolved with adequate nutrition or refeeding and may elucidate physiological and metabolic mechanisms of poor growth and life performance compared to NBW pigs later in life. (© The Author(s) 2022. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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