Protocol for the Systematic Fixation, Circuit-Based Sampling, and Qualitative and Quantitative Neuropathological Analysis of Human Brain Tissue.

Autor:	Latimer CS; University of Washington, Department of Laboratory Medicine and Pathology, Seattle, WA, USA., Melief EJ; University of Washington, Department of Laboratory Medicine and Pathology, Seattle, WA, USA., Ariza-Torres J; University of Washington, Department of Laboratory Medicine and Pathology, Seattle, WA, USA., Howard K; University of Washington, Department of Laboratory Medicine and Pathology, Seattle, WA, USA., Keen AR; University of Washington, Department of Laboratory Medicine and Pathology, Seattle, WA, USA., Keene LM; University of Washington, Department of Laboratory Medicine and Pathology, Seattle, WA, USA., Schantz AM; University of Washington, Department of Laboratory Medicine and Pathology, Seattle, WA, USA., Sytsma TM; University of Washington, Department of Laboratory Medicine and Pathology, Seattle, WA, USA., Wilson AM; University of Washington, Department of Laboratory Medicine and Pathology, Seattle, WA, USA., Grabowski TJ; University of Washington, Department of Radiology, Seattle, WA, USA., Darvas M; University of Washington, Department of Laboratory Medicine and Pathology, Seattle, WA, USA., O'Connor KD; Mount Sinai Hospital, Department of Neurology, New York, NY, USA., Nolan AL; University of Washington, Department of Laboratory Medicine and Pathology, Seattle, WA, USA., Edlow BL; Massachusetts General Hospital, Department of Neurology, Boston, MA, USA., Mac Donald CL; University of Washington, Department of Neurological Surgery, Seattle, WA, USA., Keene CD; University of Washington, Department of Laboratory Medicine and Pathology, Seattle, WA, USA. cdkeene@uw.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2561, pp. 3-30.
DOI:	10.1007/978-1-0716-2655-9_1
Abstrakt:	Human brain tissue has long been a critical resource for neuroanatomy and neuropathology, but with the advent of advanced imaging and molecular sequencing techniques, it has become possible to use human brain tissue to study, in great detail, the structural, molecular, and even functional underpinnings of human brain disease. In the century following the first description of Alzheimer's disease (AD), numerous technological advances applied to human tissue have enabled novel diagnostic approaches using diverse physical and molecular biomarkers, and many drug therapies have been tested in clinical trials (Schachter and Davis, Dialogues Clin Neurosci 2:91-100, 2000). The methods for brain procurement and tissue stabilization have remained somewhat consistently focused on formalin fixation and freezing. Although these methods have enabled research protocols of multiple modalities, new, more advanced technologies demand improved methodologies for the procurement, characterization, stabilization, and preparation of both normal and diseased human brain tissues. Here, we describe our current protocols for the procurement and characterization of fixed brain tissue, to enable systematic and precisely targeted diagnoses, and describe the novel, quantitative molecular, and neuroanatomical studies that broadly expand the use of formalin-fixed, paraffin-embedded (FFPE) tissue that will further our understanding of the mechanisms underlying human neuropathologies. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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