Harmonization of indirect reference intervals calculation by the Bhattacharya method.

Autor:	Martinez-Sanchez L; Biochemistry Department, Clinical Laboratories, Vall d'Hebron University Hospital, Barcelona, Spain.; Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Spain.; Department of Clinical Chemistry and Laboratory Medicine, Leiden University Medical Centre, Leiden, The Netherlands.; Clinical Biochemistry Research Team, Vall d'Hebron Institute of Research (VHIR), Barcelona, Spain., Gabriel-Medina P; Biochemistry Department, Clinical Laboratories, Vall d'Hebron University Hospital, Barcelona, Spain.; Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Spain.; Clinical Biochemistry Research Team, Vall d'Hebron Institute of Research (VHIR), Barcelona, Spain., Villena-Ortiz Y; Biochemistry Department, Clinical Laboratories, Vall d'Hebron University Hospital, Barcelona, Spain.; Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Spain.; Clinical Biochemistry Research Team, Vall d'Hebron Institute of Research (VHIR), Barcelona, Spain., García-Fernández AE; Biochemistry Department, Clinical Laboratories, Vall d'Hebron University Hospital, Barcelona, Spain.; Clinical Biochemistry Research Team, Vall d'Hebron Institute of Research (VHIR), Barcelona, Spain., Blanco-Grau A; Biochemistry Department, Clinical Laboratories, Vall d'Hebron University Hospital, Barcelona, Spain.; Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Spain.; Clinical Biochemistry Research Team, Vall d'Hebron Institute of Research (VHIR), Barcelona, Spain., Cobbaert CM; Department of Clinical Chemistry and Laboratory Medicine, Leiden University Medical Centre, Leiden, The Netherlands., Bravo-Nieto D; Biochemistry Department, Clinical Laboratories, Vall d'Hebron University Hospital, Barcelona, Spain.; Clinical Biochemistry Research Team, Vall d'Hebron Institute of Research (VHIR), Barcelona, Spain., Garriga-Edo S; Biochemistry Department, Clinical Laboratories, Vall d'Hebron University Hospital, Barcelona, Spain.; Clinical Biochemistry Research Team, Vall d'Hebron Institute of Research (VHIR), Barcelona, Spain., Sanz-Gea C; Biochemistry Department, Clinical Laboratories, Vall d'Hebron University Hospital, Barcelona, Spain.; Clinical Biochemistry Research Team, Vall d'Hebron Institute of Research (VHIR), Barcelona, Spain., Gonzalez-Silva G; Biochemistry Department, Clinical Laboratories, Vall d'Hebron University Hospital, Barcelona, Spain.; Clinical Biochemistry Research Team, Vall d'Hebron Institute of Research (VHIR), Barcelona, Spain., López-Hellín J; Biochemistry Department, Clinical Laboratories, Vall d'Hebron University Hospital, Barcelona, Spain.; Clinical Biochemistry Research Team, Vall d'Hebron Institute of Research (VHIR), Barcelona, Spain., Ferrer-Costa R; Biochemistry Department, Clinical Laboratories, Vall d'Hebron University Hospital, Barcelona, Spain.; Clinical Biochemistry Research Team, Vall d'Hebron Institute of Research (VHIR), Barcelona, Spain., Casis E; Clinical Laboratories, Vall d'Hebron University Hospital, Barcelona, Spain., Rodríguez-Frías F; Biochemistry Department, Clinical Laboratories, Vall d'Hebron University Hospital, Barcelona, Spain.; Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Spain.; Clinical Biochemistry Research Team, Vall d'Hebron Institute of Research (VHIR), Barcelona, Spain., den Elzen WPJ; Clinical Biochemistry Research Team, Vall d'Hebron Institute of Research (VHIR), Barcelona, Spain.; Department of Clinical Chemistry, Amsterdam UMC, University of Amsterdam, Amsterdam Public Health Research Institute, Amsterdam, The Netherlands.
Jazyk:	angličtina
Zdroj:	Clinical chemistry and laboratory medicine [Clin Chem Lab Med] 2022 Nov 17; Vol. 61 (2), pp. 266-274. Date of Electronic Publication: 2022 Nov 17 (Print Publication: 2023).
DOI:	10.1515/cclm-2022-0439
Abstrakt:	Objectives: The aim of this study was to harmonize the criteria for the Bhattacharya indirect method Microsoft Excel Spreadsheet for reference intervals calculation to reduce between-user variability and use these criteria to calculate and evaluate reference intervals for eight analytes in two different years. Methods: Anonymized laboratory test results from outpatients were extracted from January 1st 2018 to December 31st 2019. To assure data quality, we examined the monthly results from an external quality control program. Reference intervals were determined by the Bhattacharya method with the St Vincent's hospital Spreadsheet firstly using original criteria and then using additional harmonized criteria defined in this study. Consensus reference intervals using the additional harmonized criteria were calculated as the mean of four users' lower and upper reference interval results. To further test the operation criteria and robustness of the obtained reference intervals, an external user validated the Spreadsheet procedure. Results: The extracted test results for all selected laboratory tests fulfilled the quality criteria and were included in the present study. Differences between users in calculated reference intervals were frequent when using the Spreadsheet. Therefore, additional criteria for the Spreadsheet were proposed and applied by independent users, such as: to set central bin as the mean of all the data, bin size as small as possible, at least three consecutive bins and a high proportion of bins within the curve. Conclusions: The proposed criteria contributed to the harmonization of reference interval calculation between users of the Bhattacharya indirect method Spreadsheet. (© 2022 Walter de Gruyter GmbH, Berlin/Boston.)
Databáze:	MEDLINE
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