Selective TLR4 Antagonism Prevents and Reverses Morphine-Induced Persistent Postoperative Cognitive Dysfunction, Dysregulation of Synaptic Elements, and Impaired BDNF Signaling in Aged Male Rats.

Autor:	Muscat SM; Institute for Behavioral Medicine Research, The Ohio State University, Columbus, Ohio 43210.; Biomedical Sciences Graduate Program, The Ohio State University, Columbus, Ohio 43210., Deems NP; Institute for Behavioral Medicine Research, The Ohio State University, Columbus, Ohio 43210.; Neuroscience Graduate Program, The Ohio State University, Columbus, Ohio 43210., Butler MJ; Institute for Behavioral Medicine Research, The Ohio State University, Columbus, Ohio 43210., Scaria EA; Institute for Behavioral Medicine Research, The Ohio State University, Columbus, Ohio 43210., Bettes MN; Institute for Behavioral Medicine Research, The Ohio State University, Columbus, Ohio 43210., Cleary SP; Campus Chemical Instrumentation Center, The Ohio State University, Columbus, Ohio 43210., Bockbrader RH; Pharmaceutical Sciences Graduate Program, Division of Medicinal Chemistry and Pharmacognosy, The Ohio State University, Columbus, Ohio 43210., Maier SF; Department of Psychology and Neuroscience, Center for Neuroscience, University of Colorado Boulder, Boulder, Colorado 80309., Barrientos RM; Institute for Behavioral Medicine Research, The Ohio State University, Columbus, Ohio 43210 ruth.barrientos@osumc.edu.; Department of Psychiatry and Behavioral Health, The Ohio State University, Columbus, Ohio 43210.; Department of Neuroscience, The Ohio State University, Columbus, Ohio 43210.; Chronic Brain Injury Program, The Ohio State University, Columbus, Ohio 43210.
Jazyk:	angličtina
Zdroj:	The Journal of neuroscience : the official journal of the Society for Neuroscience [J Neurosci] 2023 Jan 04; Vol. 43 (1), pp. 155-172. Date of Electronic Publication: 2022 Nov 16.
DOI:	10.1523/JNEUROSCI.1151-22.2022
Abstrakt:	Perioperative neurocognitive disorders (PNDs) are characterized by confusion, difficulty with executive function, and episodic memory impairment in the hours to months following a surgical procedure. Postoperative cognitive dysfunction (POCD) represents such impairments that last beyond 30 d postsurgery and is associated with increased risk of comorbidities, progression to dementia, and higher mortality. While it is clear that neuroinflammation plays a key role in PND development, what factors underlie shorter self-resolving versus persistent PNDs remains unclear. We have previously shown that postoperative morphine treatment extends POCD from 4 d (without morphine) to at least 8 weeks (with morphine) in aged male rats, and that this effect is likely dependent on the proinflammatory capabilities of morphine via activation of toll-like receptor 4 (TLR4). Here, we extend these findings to show that TLR4 blockade, using the selective TLR4 antagonist lipopolysaccharide from the bacterium Rhodobacter sphaeroides (LPS-RS Ultrapure), ameliorates morphine-induced POCD in aged male rats. Using either a single central preoperative treatment or a 1 week postoperative central treatment regimen, we demonstrate that TLR4 antagonism (1) prevents and reverses the long-term memory impairment associated with surgery and morphine treatment, (2) ameliorates morphine-induced dysregulation of the postsynaptic proteins postsynaptic density 95 and synaptopodin, (3) mitigates reductions in mature BDNF, and (4) prevents decreased activation of the BDNF receptor TrkB (tropomyosin-related kinase B), all at 4 weeks postsurgery. We also reveal that LPS-RS Ultrapure likely exerts its beneficial effects by preventing endogenous danger signal HMGB1 (high-mobility group box 1) from activating TLR4, rather than by blocking continuous activation by morphine or its metabolites. These findings suggest TLR4 as a promising therapeutic target to prevent or treat PNDs. SIGNIFICANCE STATEMENT With humans living longer than ever, it is crucial that we identify mechanisms that contribute to aging-related vulnerability to cognitive impairment. Here, we show that the innate immune receptor toll-like receptor 4 (TLR4) is a key mediator of cognitive dysfunction in aged rodents following surgery and postoperative morphine treatment. Inhibition of TLR4 both prevented and reversed surgery plus morphine-associated memory impairment, dysregulation of synaptic elements, and reduced BDNF signaling. Together, these findings implicate TLR4 in the development of postoperative cognitive dysfunction, providing mechanistic insight and novel therapeutic targets for the treatment of cognitive impairments following immune challenges such as surgery in older individuals. (Copyright © 2023 the authors.)
Databáze:	MEDLINE
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