Development of a novel method for multiple phytohormone analysis by UHPLC-MS/MS from bio-enriched organic fertilizer prepared using banana pseudostem sap waste.

Autor: Vaidya H; Soil and Water Management Research Unit, Navsari Agricultural University, Near Eru Junction, Navsari, 396450, Gujarat, India. hariharbvaidya@gmail.com., Solanki VH; Food Quality Testing Laboratory, Navsari Agricultural University, Navsari, 396450, Gujarat, India., Kansara RV; Food Quality Testing Laboratory, Navsari Agricultural University, Navsari, 396450, Gujarat, India., Desai C; Soil and Water Management Research Unit, Navsari Agricultural University, Near Eru Junction, Navsari, 396450, Gujarat, India., Singh S; Food Quality Testing Laboratory, Navsari Agricultural University, Navsari, 396450, Gujarat, India., Patel J; Soil and Water Management Research Unit, Navsari Agricultural University, Near Eru Junction, Navsari, 396450, Gujarat, India., Vyas TK; Food Quality Testing Laboratory, Navsari Agricultural University, Navsari, 396450, Gujarat, India., Patel H; School of Sciences, P.P. Savani University, Surat, 394135, Gujarat, India.
Jazyk: angličtina
Zdroj: Environmental science and pollution research international [Environ Sci Pollut Res Int] 2023 Jun; Vol. 30 (28), pp. 71482-71490. Date of Electronic Publication: 2022 Nov 15.
DOI: 10.1007/s11356-022-23941-6
Abstrakt: Banana harvesting generates a large amount of banana pseudostem waste, which is generally burnt or thrown away, despite containing many nutrients. Bio-enriched organic fertilizer (BOF) was prepared from banana pseudostem sap (BPS), and it has been patented (Patent No. WO 2013/001478 Al). Several reports revealed that its application increases plant growth promotion of various horticulture crops. Apart from macro- and micronutrients, it also contained phytohormones. Hence, the present study aims to detect and quantify phytohormone in it. A novel method was developed to extract four phytohormones, viz., indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), gibberellic acid (GA 3 ), and salicylic acid (SA) using single solvent from BPS and BOF. Extracted hormones were analyzed by ultrahigh-performance liquid chromatography coupled with heated electrospray ionization tandem mass spectrometry (UHPLC-HESI-MS/MS). BOF showed a higher concentration of IAA, IBA, GA 3 , and SA than BPS. Thus, this is the first time a method has been reported to extract and detect phytohormones from banana pseudostem sap.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: