Mobile resistome of microbial communities and antimicrobial residues from drinking water supply systems in Rio de Janeiro, Brazil.

Autor: Bianco K; Instituto Nacional de Controle de Qualidade Em Saúde INCQS/FIOCRUZ, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, 4365, Brazil. kayo.bianco@incqs.fiocruz.br., de Farias BO; Instituto Nacional de Controle de Qualidade Em Saúde INCQS/FIOCRUZ, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, 4365, Brazil., Gonçalves-Brito AS; Instituto Nacional de Controle de Qualidade Em Saúde INCQS/FIOCRUZ, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, 4365, Brazil., Alves do Nascimento AP; Instituto Nacional de Controle de Qualidade Em Saúde INCQS/FIOCRUZ, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, 4365, Brazil., Magaldi M; Instituto Nacional de Controle de Qualidade Em Saúde INCQS/FIOCRUZ, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, 4365, Brazil., Montenegro K; Instituto Nacional de Controle de Qualidade Em Saúde INCQS/FIOCRUZ, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, 4365, Brazil., Flores C; Instituto Nacional de Controle de Qualidade Em Saúde INCQS/FIOCRUZ, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, 4365, Brazil., Oliveira S; Instituto Nacional de Controle de Qualidade Em Saúde INCQS/FIOCRUZ, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, 4365, Brazil., Monteiro MA; Instituto Nacional de Controle de Qualidade Em Saúde INCQS/FIOCRUZ, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, 4365, Brazil., Spisso BF; Instituto Nacional de Controle de Qualidade Em Saúde INCQS/FIOCRUZ, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, 4365, Brazil., Pereira MU; Instituto Nacional de Controle de Qualidade Em Saúde INCQS/FIOCRUZ, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, 4365, Brazil., Ferreira RG; Instituto Nacional de Controle de Qualidade Em Saúde INCQS/FIOCRUZ, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, 4365, Brazil., Albano RM; Rio de Janeiro State University, Rio de Janeiro, Brazil., Cardoso AM; Rio de Janeiro State University, Rio de Janeiro, Brazil., Clementino MM; Instituto Nacional de Controle de Qualidade Em Saúde INCQS/FIOCRUZ, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, 4365, Brazil.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Nov 09; Vol. 12 (1), pp. 19050. Date of Electronic Publication: 2022 Nov 09.
DOI: 10.1038/s41598-022-21040-7
Abstrakt: Antibiotic resistance genes (ARGs) are widespread in the environment due to the overuse of antibiotics and other pollutants, posing a threat to human and animal health. In this study, we evaluated antimicrobial residues, bacterial diversity and ARGs in two important watersheds, Guandu and São João, that supply drinking water to Rio de Janeiro city, Brazil. In addition, tap water samples were collected from three different cities in Rio de Janeiro State, including the metropolitan area of Rio de Janeiro city. Clarithromycin, sulfamethoxazole and azithromycin were found in untreated water and drinking water in all samples. A greater abundance of Proteobacteria was observed in Guandu and São João watersheds, with most of the sequences belonging to the Gammaproteobacteria class. A plasmidome-focused metagenomics approach revealed 4881 (Guandu), 3705 (São João) and 3385 (drinking water) ARGs mainly associated with efflux systems. The genes encoding metallo-β-lactamase enzymes (bla AIM , bla GIM , bla IMP , and bla VIM ) were detected in the two watersheds and in drinking water samples. Moreover, we demonstrated the presence of the colistin resistance genes mcr-3 and mcr-4 (both watersheds) and mcr-9 (drinking water and Guandu) for the first time in Brazil. Our data emphasize the importance of introducing measures to reduce the disposal of antibiotics and other pollutants capable of promoting the occurrence and spread of the microbial resistome on aquatic environments and predicting possible negative impacts on human health.
(© 2022. The Author(s).)
Databáze: MEDLINE
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