FOXD1 is required for 3D patterning of the kidney interstitial matrix.

Autor: Lipp SN; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA.; The Indiana University Medical Scientist/Engineer Training Program, Indianapolis, Indiana, USA., Jacobson KR; Purdue University Interdisciplinary Life Science Program, Purdue University, West Lafayette, Indiana, USA., Schwaderer AL; Department of Pediatrics, Indiana University School of Medicine, Riley Children's Hospital, Indianapolis, Indiana, USA., Hains DS; Department of Pediatrics, Indiana University School of Medicine, Riley Children's Hospital, Indianapolis, Indiana, USA., Calve S; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA.; Purdue University Interdisciplinary Life Science Program, Purdue University, West Lafayette, Indiana, USA.; Department of Mechanical Engineering, University of Colorado--Boulder, Boulder, Colorado, USA.
Jazyk: angličtina
Zdroj: Developmental dynamics : an official publication of the American Association of Anatomists [Dev Dyn] 2023 Apr; Vol. 252 (4), pp. 463-482. Date of Electronic Publication: 2023 Jan 16.
DOI: 10.1002/dvdy.545
Abstrakt: Background: The interstitial extracellular matrix (ECM) is comprised of proteins and glycosaminoglycans and provides structural and biochemical information during development. Our previous work revealed the presence of transient ECM-based structures in the interstitial matrix of developing kidneys. Stromal cells are the main contributors to interstitial ECM synthesis, and the transcription factor Forkhead Box D1 (Foxd1) is critical for stromal cell function. To investigate the role of Foxd1 in interstitial ECM patterning, we combined 3D imaging and proteomics to explore how the matrix changes in the murine developing kidney when Foxd1 is knocked out.
Results: We found that COL26A1, FBN2, EMILIN1, and TNC, interstitial ECM proteins that are transiently upregulated during development, had a similar distribution perinatally but then diverged in patterning in the adult. Abnormally clustered cortical vertical fibers and fused glomeruli were observed when Foxd1 was knocked out. The changes in the interstitial ECM of Foxd1 knockout kidneys corresponded to disrupted Foxd1 + cell patterning but did not precede branching dysmorphogenesis.
Conclusions: The transient ECM networks affected by Foxd1 knockout may provide support for later-stage nephrogenic structures.
(© 2022 The Authors. Developmental Dynamics published by Wiley Periodicals LLC on behalf of American Association for Anatomy.)
Databáze: MEDLINE
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