Metabolomics profiling in acute liver transplant rejection in a pediatric population.

Autor: Frediani JK; Nell Hodgson Woodruff School of Nursing, Emory University, Atlanta, GA, USA.; Nutrition and Health Sciences, Rollins School of Public Health, Laney Graduate School, Emory University, Atlanta, GA, USA., Beyh YS; Nutrition and Health Sciences, Rollins School of Public Health, Laney Graduate School, Emory University, Atlanta, GA, USA., Gupta N; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, USA.; Transplant Services, Children's Healthcare of Atlanta, Atlanta, GA, USA., Westbrook AL; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, USA., Cleeton R; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, USA., Cordero M; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, USA., Hernandez A; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, USA., Tran V; Department of Pulmonology, Emory University School of Medicine, Atlanta, GA, USA., Jones DP; Department of Pulmonology, Emory University School of Medicine, Atlanta, GA, USA., Vos MB; Nutrition and Health Sciences, Rollins School of Public Health, Laney Graduate School, Emory University, Atlanta, GA, USA. mvos@emory.edu.; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, USA. mvos@emory.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Nov 04; Vol. 12 (1), pp. 18663. Date of Electronic Publication: 2022 Nov 04.
DOI: 10.1038/s41598-022-18957-4
Abstrakt: Pediatric liver transplantation rejection affects 20% of children. Currently, liver biopsy, expensive and invasive, is the best method of diagnosis. Discovery and validation of clinical biomarkers from blood or other biospecimens would improve clinical care. For this study, stored plasma samples were utilized from two cross-sectional cohorts of liver transplant patients at Children's Healthcare of Atlanta. High resolution metabolic profiling was completed using established methods. Children with (n = 18) or without (n = 25) acute cellular rejection were included in the analysis (n = 43 total). The mean age of these racially diverse cohorts ranged from 12.6 years in the rejection group and 13.6 years in the no rejection group. Linear regression provided 510 significantly differentiating metabolites between groups, and OPLS-DA showed 145 metabolites with VIP > 2. A total of 95 overlapping significant metabolites between OPLS-DA and linear regression analyses were detected. Pathway analysis (p < 0.05) showed bile acid biosynthesis and tryptophan metabolism as the top two differentiating pathways. Network analysis also identified tryptophan and clustered with liver enzymes and steroid use. We conclude metabolic profiling of plasma from children with acute liver transplant rejection demonstrates > 500 significant metabolites. This result suggests that development of a non-invasive biomarker-based test is possible for rejection screening.
(© 2022. The Author(s).)
Databáze: MEDLINE
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