Deep into the Apoplast: Grapevine and Plasmopara viticola Proteomes Reveal the Secret Beneath Host and Pathogen Communication at 6 h After Contact.

Autor:	Figueiredo J; Grapevine Pathogen Systems Lab, BioISI - Biosystems & Integrative Sciences Institute, Plant Biology Department, Faculty of Sciences, University of Lisbon, 1749-016 Lisboa, Portugal., Santos RB; Grapevine Pathogen Systems Lab, BioISI - Biosystems & Integrative Sciences Institute, Plant Biology Department, Faculty of Sciences, University of Lisbon, 1749-016 Lisboa, Portugal., Guerra-Guimarães L; Centro de Investigação das Ferrugens do Cafeeiro, Instituto Superior de Agronomia, Universidade de Lisboa, 1349-017 Lisboa, Portugal.; Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, 1349-017 Lisboa, Portugal., Leclercq CC; Luxembourg Institute of Science and Technology, L-4362 Esch-sur-Alzette, Luxembourg., Renaut J; Luxembourg Institute of Science and Technology, L-4362 Esch-sur-Alzette, Luxembourg., Sousa L; Departamento de Estatística e Investigação Operacional, Centro de Estatística e Aplicações, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal., Figueiredo A; Grapevine Pathogen Systems Lab, BioISI - Biosystems & Integrative Sciences Institute, Plant Biology Department, Faculty of Sciences, University of Lisbon, 1749-016 Lisboa, Portugal.
Jazyk:	angličtina
Zdroj:	Phytopathology [Phytopathology] 2023 May; Vol. 113 (5), pp. 893-903. Date of Electronic Publication: 2023 May 17.
DOI:	10.1094/PHYTO-09-22-0340-FI
Abstrakt:	The apoplast is the first hub of plant-pathogen communication where pathogen effectors are recognized by plant defensive proteins and cell receptors, thus activating signal transduction pathways. As a result of this first contact, the host triggers a defense response that involves the modulation of extra- and intracellular proteins. In grapevine-pathogen interactions, little is known about the trafficking between extra- and intracellular spaces. Grapevine is an economically important crop that relies on heavy fungicide use to control several diseases, and a deeper knowledge on the activation of its immune response is crucial to define new control strategies. In this study, we focused on the first 6 h postinoculation with Plasmopara viticola to evaluate grapevine proteome modulation in the apoplast. The in planta P. viticola proteome was also assessed to enable a deeper understanding of plant-pathogen communication. Our results showed that several plant mechanisms are triggered in the tolerant grapevine cultivar Regent after inoculation, such as oomycete recognition, plant cell wall modifications, reactive oxygen species signaling, and secretion of proteins to disrupt oomycete structures. On the other hand, P. viticola proteins related to development and virulence were the most predominant. This pioneer study highlights the early dynamics of cellular communication in grapevine defense that leads to the successful establishment of an incompatible interaction. Competing Interests: The author(s) declare no conflict of interest.
Databáze:	MEDLINE
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