Cell adhesion proteins in the cerebrospinal fluid of neonates prenatally exposed to Zika virus: A case-control study.

Autor: Ramos CL; Bahiana Foundation for Science Development, Bahiana School of Medicine, Salvador, Brazil., Nascimento-Carvalho EC; Bahiana Foundation for Science Development, Bahiana School of Medicine, Salvador, Brazil., Nascimento-Carvalho GC; Bahiana Foundation for Science Development, Bahiana School of Medicine, Salvador, Brazil., VanDuijn MM; Department of Neurology, Erasmus MC, Rotterdam, Netherlands., Vilas-Boas AL; Bahiana Foundation for Science Development, Bahiana School of Medicine, Salvador, Brazil., Moreno-Carvalho OA; Cerebrospinal Fluid Laboratory, José Silveira Foundation, Salvador, Brazil., Carvalho LP; Laboratory of Clinical Research, LAPEC, Gonçalo Moniz Institute, Salvador, Brazil., Zeneyedpour L; Department of Neurology, Erasmus MC, Rotterdam, Netherlands., Ferwerda G; Section of Paediatric Infectious Diseases, Laboratory of Medical Immunology, Radboud Institute for Molecular Life Sciences, Radboud Centre for Infectious Diseases, Radboud University Medical Center, Nijmegen, Netherlands., de Groot R; Section of Paediatric Infectious Diseases, Laboratory of Medical Immunology, Radboud Institute for Molecular Life Sciences, Radboud Centre for Infectious Diseases, Radboud University Medical Center, Nijmegen, Netherlands., Luider TM; Department of Neurology, Erasmus MC, Rotterdam, Netherlands., Nascimento-Carvalho CM; Department of Paediatrics, Federal University of Bahia School of Medicine, Salvador, Brazil.
Jazyk: angličtina
Zdroj: The European journal of neuroscience [Eur J Neurosci] 2022 Dec; Vol. 56 (12), pp. 6258-6268. Date of Electronic Publication: 2022 Nov 14.
DOI: 10.1111/ejn.15851
Abstrakt: To compare cell adhesion molecules levels in cerebrospinal fluid (CSF) between Zika virus (ZIKV)-exposed neonates with/without microcephaly (cases) and controls, 16 neonates (cases), 8 (50%) with and 8 (50%) without microcephaly, who underwent lumbar puncture (LP) during the ZIKV epidemic (2015-2016) were included. All mothers reported ZIKV clinical symptoms during gestation, all neonates presented with congenital infection findings, and other congenital infections were ruled out. Fourteen control neonates underwent LP in the same laboratory (2017-2018). Five cell adhesion proteins were measured in the CSF using mass spectrometry. Neurexin-1 (3.50 [2.00-4.00] vs. 7.5 [5.00-10.25], P = 0.001), neurexin-3 (0.00 [0.00-0.00] vs. 3.00 [1.50-4.00], P = 0.001) and neural cell adhesion molecule 2 (NCAM2) (0.00 [0.00-0.75] vs. 1.00 [1.00-2.00], P = 0.001) were significantly lower in microcephalic and non-microcephalic cases than in controls. When these two sub-groups of prenatally ZIKA-exposed children were compared to controls separately, the same results were found. When cases with and without microcephaly were compared, no difference was found. Neurexin-3 (18.8% vs. 78.6%, P = 0.001) and NCAM2 (25.0% vs. 85.7%, P = 0.001) were less frequently found among the cases. A positive correlation was found between cephalic perimeter and levels of these two proteins. Neurexin-2 and neurexin-2b presented no significant differences. Levels of three cell adhesion proteins were significantly lower in CSF of neonates exposed to ZIKV before birth than in controls, irrespective of presence of congenital microcephaly. Moreover, the smaller the cephalic perimeter, the lower CSF cell adhesion protein levels. These findings suggest that low CSF levels of neurexin-1, neurexin-3 and NCAM2 may reflect the effects of ZIKV on foetal brain development.
(© 2022 Federation of European Neuroscience Societies and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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