| Autor: |
Alzoughool F; Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, The Hashemite University, Zarqa 13133, Jordan.; Division of Health Sciences, Fujairah Women's College, Higher Colleges of Technology, Fujairah 1626, United Arab Emirates., Al-Zghoul MB; Basic Veterinary Sciences, School of Veterinary Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan., Ghanim BY; University of Petra Pharmaceutical Center (UPPC), University of Petra, Amman 11196, Jordan., Atoum M; Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, The Hashemite University, Zarqa 13133, Jordan., Aljawarneh Y; Division of Health Sciences, Fujairah Women's College, Higher Colleges of Technology, Fujairah 1626, United Arab Emirates., Idkaidek N; Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy and Medical Sciences, University of Petra, Amman 11196, Jordan., Qinna NA; University of Petra Pharmaceutical Center (UPPC), University of Petra, Amman 11196, Jordan.; Department of Pharmacology and Biomedical Sciences, Faculty of Pharmacy and Medical Sciences, University of Petra, Amman 11196, Jordan. |
| Abstrakt: |
Irisin is an exercise-induced myokine implicated as a fundamental mediator of physical activity benefits. The aim of the present study was to investigate the role of the chronic administration model of irisin on the physiological and molecular status of skeletal muscle. A total of 20 female Sprague Dawley rats (250 ± 40 g) were implanted with an irisin-loaded osmotic pump (5 µg/kg/day) for 42 days; in addition, 3 females received a single subcutaneous injection of irisin (5 µg/kg). On a weekly basis for six weeks, animals were weighed and blood samples were collected. After 42 days, hind muscle biopsies were collected for histology and gene analysis. Serum irisin, clinical biochemistry, and histopathology were quantified and evaluated. Genes encoding for different physiological muscle activities, such as oxidative stress, fatty acid metabolism, muscle hypertrophy, mitochondrial fusion, and aging were assayed. The results showed a significant reduction in body weight percentage and creatine kinase level without affecting the morphological characteristics of skeletal muscle. Significant changes were noted in genes involved in muscle physiological activity, growth, and aging, as well as genes encoding for the antioxidant system, fatty acid oxidation processes, and mitochondrial fusion. In conclusion, exogenous irisin can induce the same physiological and molecular mechanisms that might be induced by exercise. |
