The translation regulator Zar1l controls timing of meiosis in Xenopus oocytes.

Autor:	Heim A; Department of Biology, University of Konstanz, 78457 Konstanz, Germany., Niedermeier ML; Department of Biology, University of Konstanz, 78457 Konstanz, Germany.; Konstanz Research School Chemical Biology, University of Konstanz, 78457 Konstanz, Germany., Stengel F; Department of Biology, University of Konstanz, 78457 Konstanz, Germany.; Konstanz Research School Chemical Biology, University of Konstanz, 78457 Konstanz, Germany., Mayer TU; Department of Biology, University of Konstanz, 78457 Konstanz, Germany.; Konstanz Research School Chemical Biology, University of Konstanz, 78457 Konstanz, Germany.
Jazyk:	angličtina
Zdroj:	Development (Cambridge, England) [Development] 2022 Nov 01; Vol. 149 (21). Date of Electronic Publication: 2022 Oct 24.
DOI:	10.1242/dev.200900
Abstrakt:	Oocyte maturation and early embryo development occur in vertebrates in the near absence of transcription. Thus, sexual reproduction of vertebrates critically depends on the timely translation of mRNAs already stockpiled in the oocyte. Yet how translational activation of specific mRNAs is temporally coordinated is still incompletely understood. Here, we elucidate the function of Zar1l, a yet uncharacterized member of the Zar RNA-binding protein family, in Xenopus oocytes. Employing TRIM-Away, we demonstrate that loss of Zar1l accelerates hormone-induced meiotic resumption of Xenopus oocytes due to premature accumulation of the M-phase-promoting kinase cMos. We show that Zar1l is a constituent of a large ribonucleoparticle containing the translation repressor 4E-T and the central polyadenylation regulator CPEB1, and that it binds directly to the cMos mRNA. Partial, hormone-induced degradation of Zar1l liberates 4E-T from CPEB1, which weakens translational repression of mRNAs encoding cMos and likely additional M-phase-promoting factors. Thus, our study provides fundamental insights into the mechanisms that ensure temporally regulated translation of key cell cycle regulators during oocyte maturation, which is essential for sexual reproductivity. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2022. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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