Diverse MarR bacterial regulators of auxin catabolism in the plant microbiome.

Autor: Conway JM; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ, USA., Walton WG; Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Salas-González I; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Curriculum in Bioinformatics and Computational Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Law TF; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Lindberg CA; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Crook LE; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Kosina SM; Environmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Fitzpatrick CR; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Lietzan AD; Division of Oral and Craniofacial Health Sciences, Adams School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Northen TR; Environmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.; Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Jones CD; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Curriculum in Bioinformatics and Computational Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Finkel OM; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Department of Plant and Environmental Sciences, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel., Redinbo MR; Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. redinbo@unc.edu.; Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. redinbo@unc.edu.; Department of Biochemistry and Biophysics, and the Integrative Program for Biological and Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. redinbo@unc.edu.; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. redinbo@unc.edu., Dangl JL; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. dangl@email.unc.edu.; Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. dangl@email.unc.edu.; Curriculum in Bioinformatics and Computational Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. dangl@email.unc.edu.; Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. dangl@email.unc.edu.; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. dangl@email.unc.edu.
Jazyk: angličtina
Zdroj: Nature microbiology [Nat Microbiol] 2022 Nov; Vol. 7 (11), pp. 1817-1833. Date of Electronic Publication: 2022 Oct 20.
DOI: 10.1038/s41564-022-01244-3
Abstrakt: Chemical signalling in the plant microbiome can have drastic effects on microbial community structure, and on host growth and development. Previously, we demonstrated that the auxin metabolic signal interference performed by the bacterial genus Variovorax via an auxin degradation locus was essential for maintaining stereotypic root development in an ecologically relevant bacterial synthetic community. Here, we dissect the Variovorax auxin degradation locus to define the genes iadDE as necessary and sufficient for indole-3-acetic acid (IAA) degradation and signal interference. We determine the crystal structures and binding properties of the operon's MarR-family repressor with IAA and other auxins. Auxin degradation operons were identified across the bacterial tree of life and we define two distinct types on the basis of gene content and metabolic products: iac-like and iad-like. The structures of MarRs from representatives of each auxin degradation operon type establish that each has distinct IAA-binding pockets. Comparison of representative IAA-degrading strains from diverse bacterial genera colonizing Arabidopsis plants show that while all degrade IAA, only strains containing iad-like auxin-degrading operons interfere with auxin signalling in a complex synthetic community context. This suggests that iad-like operon-containing bacterial strains, including Variovorax species, play a key ecological role in modulating auxins in the plant microbiome.
(© 2022. The Author(s).)
Databáze: MEDLINE
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