| Autor: |
Nifant'ev I; A.V. Topchiev Institute of Petrochemical Synthesis RAS, 29 Leninsky Pr., 119991 Moscow, Russia.; Chemistry Department, M.V. Lomonosov Moscow State University, 1-3 Leninskie Gory, 119991 Moscow, Russia.; Chemistry Department, National Research University Higher School of Economics, 20 Miasnitskaya Street, 101000 Moscow, Russia., Besprozvannykh V; A.V. Topchiev Institute of Petrochemical Synthesis RAS, 29 Leninsky Pr., 119991 Moscow, Russia.; Chemistry Department, National Research University Higher School of Economics, 20 Miasnitskaya Street, 101000 Moscow, Russia., Shlyakhtin A; A.V. Topchiev Institute of Petrochemical Synthesis RAS, 29 Leninsky Pr., 119991 Moscow, Russia.; Chemistry Department, M.V. Lomonosov Moscow State University, 1-3 Leninskie Gory, 119991 Moscow, Russia., Tavtorkin A; A.V. Topchiev Institute of Petrochemical Synthesis RAS, 29 Leninsky Pr., 119991 Moscow, Russia., Legkov S; A.V. Topchiev Institute of Petrochemical Synthesis RAS, 29 Leninsky Pr., 119991 Moscow, Russia., Chinova M; A.V. Topchiev Institute of Petrochemical Synthesis RAS, 29 Leninsky Pr., 119991 Moscow, Russia., Arutyunyan I; Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, 4 Oparin Street, 117997 Moscow, Russia.; Institute of Medicine, Peoples' Friendship University of Russia, Miklukho-Maklaya 6 Street, 117198 Moscow, Russia., Soboleva A; Institute of Medicine, Peoples' Friendship University of Russia, Miklukho-Maklaya 6 Street, 117198 Moscow, Russia.; Research Institute of Human Morphology, 3 Tsyurupy Street, 117418 Moscow, Russia., Fatkhudinov T; Institute of Medicine, Peoples' Friendship University of Russia, Miklukho-Maklaya 6 Street, 117198 Moscow, Russia.; Research Institute of Human Morphology, 3 Tsyurupy Street, 117418 Moscow, Russia., Ivchenko P; A.V. Topchiev Institute of Petrochemical Synthesis RAS, 29 Leninsky Pr., 119991 Moscow, Russia.; Chemistry Department, M.V. Lomonosov Moscow State University, 1-3 Leninskie Gory, 119991 Moscow, Russia. |
| Abstrakt: |
Composite biocompatible scaffolds, obtained using the electrospinning (ES) technique, are highly promising for biomedical application thanks to their high surface area, porosity, adjustable fiber diameter, and permeability. However, the combination of synthetic biodegradable (such as poly(ε-caprolactone) PCL) and natural (such as gelatin Gt) polymers is complicated by the problem of low compatibility of the components. Previously, this problem was solved by PCL grafting and/or Gt cross-linking after ES molding. In the present study, composite fibrous scaffolds consisting of PCL and Gt were fabricated by the electrospinning (ES) method using non-functionalized PCL1 or NHS-functionalized PCL2 and hexafluoroisopropanol as a solvent. To provide covalent binding between PCL2 and Gt macromolecules, NHS-functionalized methyl glutarate was synthesized and studied in model reactions with components of spinning solution. It was found that selective formation of amide bonds, which provide complete covalent bonding of Gt in PCL/Gt composite, requires the presence of weak acid. With the use of the optimized ES method, fibrous mats with different PCL/Gt ratios were prepared. The sample morphology (SEM), hydrolytic resistance (FT-IR), cell adhesion and viability (MTT assay), cell penetration (fluorescent microscopy), and mechanical characteristics of the samples were studied. PCL2 -based films with a Gt content of 20 wt% have demonstrated the best set of properties. |
