| Autor: |
de Moraes Segundo JDP; Department of Chemical Engineering, Federal University of Ceará, Fortaleza 60455-760, Brazil.; Department of Materials and Bioprocess Engineering, University of Campinas, Campinas 13083-852, Brazil.; Department of Manufacturing and Materials Engineering, University of Campinas, Campinas 13083-860, Brazil., Constantino JSF; Department of Chemical Engineering, Federal University of Ceará, Fortaleza 60455-760, Brazil., Calais GB; Department of Materials and Bioprocess Engineering, University of Campinas, Campinas 13083-852, Brazil., de Moura Junior CF; Department of Materials and Bioprocess Engineering, University of Campinas, Campinas 13083-852, Brazil., de Moraes MOS; Thematic Laboratory of Microscopy and Nanotechnology, National Institute of Amazonian Research, Manaus 69067-001, Brazil., da Fonseca JHL; Department of Manufacturing and Materials Engineering, University of Campinas, Campinas 13083-860, Brazil., Tsukamoto J; Department of Genetics, Evolution, Microbiology and Immunology, University of Campinas, Campinas 13083-970, Brazil., Monteiro RRC; Department of Chemical Engineering, Federal University of Ceará, Fortaleza 60455-760, Brazil., Andrade FK; Department of Chemical Engineering, Federal University of Ceará, Fortaleza 60455-760, Brazil., d'Ávila MA; Department of Manufacturing and Materials Engineering, University of Campinas, Campinas 13083-860, Brazil., Arns CW; Department of Genetics, Evolution, Microbiology and Immunology, University of Campinas, Campinas 13083-970, Brazil., Beppu MM; Department of Materials and Bioprocess Engineering, University of Campinas, Campinas 13083-852, Brazil., Vieira RS; Department of Chemical Engineering, Federal University of Ceará, Fortaleza 60455-760, Brazil. |
| Abstrakt: |
Electrospinning technology was used to produced polyvinylpyrrolidone (PVP)-copper salt composites with structural differences, and their virucidal activity against coronavirus was investigated. The solutions were prepared with 20, 13.3, 10, and 6.6% w / v PVP containing 3, 1.0, 0.6, and 0.2% w / v Cu (II), respectively. The rheological properties and electrical conductivity contributing to the formation of the morphologies of the composite materials were observed by scanning electron microscopy (SEM). SEM images revealed the formation of electrospun PVP-copper salt ultrafine composite fibers (0.80 ± 0.35 µm) and electrosprayed PVP-copper salt composite microparticles (1.50 ± 0.70 µm). Energy-dispersive X-ray spectroscopy (EDS) evidenced the incorporation of copper into the produced composite materials. IR spectra confirmed the chemical composition and showed an interaction of Cu (II) ions with oxygen in the PVP resonant ring. Virucidal composite fibers inactivated 99.999% of coronavirus within 5 min of contact time, with moderate cytotoxicity to L929 cells, whereas the virucidal composite microparticles presented with a virucidal efficiency of 99.999% within 1440 min of exposure, with low cytotoxicity to L929 cells (mouse fibroblast). This produced virucidal composite materials have the potential to be applied in respirators, personal protective equipment, self-cleaning surfaces, and to fabric coat personal protective equipment against SARS-CoV-2, viral outbreaks, or pandemics. |
