Generation of three human iPSC lines from patients with Huntington's disease with different CAG lengths and human control iPSC line from a healthy donor.

Autor:	Latoszek E; Laboratory of Neurodegeneration, International Institute of Molecular and Cell Biology in Warsaw, Poland., Piechota M; Laboratory of Neurodegeneration, International Institute of Molecular and Cell Biology in Warsaw, Poland., Liszewska E; Laboratory of Molecular and Cellular Neurobiology, International Institute of Molecular and Cell Biology in Warsaw, Poland., Hansíková H; Laboratory for Study of Mitochondrial Disorders, Department of Pediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic., Klempíř J; Department of Neurology and Center of Clinical Neuroscience, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic., Mühlbäck A; Department of Neurology and Center of Clinical Neuroscience, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic; Department of Neurology, Ulm University, Germany., Landwehrmeyer GB; Department of Neurology, Ulm University, Germany., Kuznicki J; Laboratory of Neurodegeneration, International Institute of Molecular and Cell Biology in Warsaw, Poland., Czeredys M; Laboratory of Neurodegeneration, International Institute of Molecular and Cell Biology in Warsaw, Poland. Electronic address: mczeredys@iimcb.gov.pl.
Jazyk:	angličtina
Zdroj:	Stem cell research [Stem Cell Res] 2022 Oct; Vol. 64, pp. 102931. Date of Electronic Publication: 2022 Oct 03.
DOI:	10.1016/j.scr.2022.102931
Abstrakt:	Huntington's disease (HD) is a progressive neurodegenerative disorder with autosomal-dominant heritability that affect the central nervous system and peripheral tissues. The human-induced pluripotent stem cells (hiPSC) lines were generated from dermal fibroblasts of patients without comorbidities, non-smokers, at the pre-manifest (IIMCBi004-A), early-manifest (IIMCBi005-A), and manifest (IIMCBi006-A) HD stage assessed by neurological tests, as well as from a healthy donor (IIMCBi003-A). Characterization showed that the obtained hiPSC lines contained different CAG repeats consistent with the number of CAG repeats in original fibroblasts. Moreover, hiPSCs expressed pluripotency markers and were able to differentiate into three-germ layers in vitro. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2022. Published by Elsevier B.V.)
Databáze:	MEDLINE
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