Effect of Poly(Oxanorbonene)- and Poly(Methacrylate)-Based Polyzwitterionic Surface Coatings on Cell Adhesion and Gene Expression of Human Keratinocytes.

Autor: Eickenscheidt A; Department of Microsystems Engineering (IMTEK), University of Freiburg, Georges-Köhler-Allee 103, 79110, Freiburg, Germany.; Freiburg Center for Interactive Materials and Bioinspired Technologies (FIT), University of Freiburg, Georges-Köhler-Allee 105, 79110, Freiburg, Germany., Lavaux V; Department of Microsystems Engineering (IMTEK), University of Freiburg, Georges-Köhler-Allee 103, 79110, Freiburg, Germany.; Freiburg Center for Interactive Materials and Bioinspired Technologies (FIT), University of Freiburg, Georges-Köhler-Allee 105, 79110, Freiburg, Germany., Paschke S; Department of Microsystems Engineering (IMTEK), University of Freiburg, Georges-Köhler-Allee 103, 79110, Freiburg, Germany.; Freiburg Center for Interactive Materials and Bioinspired Technologies (FIT), University of Freiburg, Georges-Köhler-Allee 105, 79110, Freiburg, Germany., Martínez AG; Institut für Chemie, Universität Potsdam, Karl-Liebknecht Str. 25, 14476, Potsdam-Golm, Germany., Schönemann E; Institut für Chemie, Universität Potsdam, Karl-Liebknecht Str. 25, 14476, Potsdam-Golm, Germany., Laschewsky A; Institut für Chemie, Universität Potsdam, Karl-Liebknecht Str. 25, 14476, Potsdam-Golm, Germany.; Fraunhofer Institut für Angewandte Polymerforschung, 14476, Potsdam-Golm, Germany., Lienkamp K; Department of Materials Science, Saarland University, Campus, 66123, Saarbrücken, Germany., Staszewski O; Institute for Neuropathology, Medical Center of the University of Freiburg, Hugstetter Str. 55, 79106, Freiburg, Germany.
Jazyk: angličtina
Zdroj: Macromolecular bioscience [Macromol Biosci] 2022 Nov; Vol. 22 (11), pp. e2200225. Date of Electronic Publication: 2022 Oct 17.
DOI: 10.1002/mabi.202200225
Abstrakt: Polyzwitterions are generally known for their anti-adhesive properties, including resistance to protein and cell adhesion, and overall high bio-inertness. Yet there are a few polyzwitterions to which mammalian cells do adhere. To understand the structural features of this behavior, a panel of polyzwitterions with different functional groups and overall degrees of hydrophobicity is analyzed here, and their physical and biological properties are correlated to these structural differences. Cell adhesion is focused on, which is the basic requirement for cell viability, proliferation, and growth. With the here presented polyzwitterion panel, three different types of cell-surface interactions are observed: adhesion, slight attachment, and cell repellency. Using immunofluorescence methods, it is found that human keratinocytes (HaCaT) form focal adhesions on the cell-adhesive polyzwitterions, but not on the sample that has only slight cell attachment. Gene expression analysis indicates that HaCaT cells cultivated in the presence of a non-adhesive polyzwitterion have up-regulated inflammatory and apoptosis-related cell signaling pathways, while the gene expression of HaCaT cells grown on a cell-adhesive polyzwitterion does not differ from the gene expression of the growth control, and thus can be defined as fully cell-compatible.
(© 2022 The Authors. Macromolecular Bioscience published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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